Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)
Published Online: 11 MAR 2004
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Graham, J. M. and Rickwood, D. 2004. Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation). eLS. .
- Published Online: 11 MAR 2004
Mononuclear cells (lymphocytes and monocytes) can be isolated from other blood cell elements on the basis of their lower density. There are three variations of the basic method: (1) sedimentation on to a density barrier using diatrizoate (plus a polysaccharide) or Nycodenz or iodixanol; (2) flotation after raising the density of the plasma by mixing blood with Nycodenz or iodixanol; (3) flotation through an iodixanol density barrier.
- peripheral blood;
- mononuclear cell separation;
- cell separation