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Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation)

  1. John M Graham1,
  2. David Rickwood2

Published Online: 11 MAR 2004

DOI: 10.1038/npg.els.0003801

eLS

eLS

How to Cite

Graham, J. M. and Rickwood, D. 2004. Isolation of Mononuclear Cells from Human Blood (Density Barrier Sedimentation). eLS. .

Author Information

  1. 1

    Wirral, UK

  2. 2

    University of Essex, Colchester, UK

Publication History

  1. Published Online: 11 MAR 2004

Abstract

Mononuclear cells (lymphocytes and monocytes) can be isolated from other blood cell elements on the basis of their lower density. There are three variations of the basic method: (1) sedimentation on to a density barrier using diatrizoate (plus a polysaccharide) or Nycodenz or iodixanol; (2) flotation after raising the density of the plasma by mixing blood with Nycodenz or iodixanol; (3) flotation through an iodixanol density barrier.

Keywords:

  • peripheral blood;
  • human;
  • lymphocytes;
  • monocytes;
  • mononuclear cell separation;
  • cell separation