Ligation: Theory and Practice
Published Online: 23 SEP 2005
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Kandavelou, K., Mani, M., Reddy, S. P. and Chandrasegaran, S. 2005. Ligation: Theory and Practice. eLS. .
- Published Online: 23 SEP 2005
Ligation is the process by which NAD DNA ligases catalyse the formation of phosphodiester bonds between juxtaposed 3′-hydroxyl group and 5′-phosphate termini in duplex deoxyribonucleic acid (DNA). Ligases use adenosine triphosphate or nicotinamide–adenine dinucleotide (NAD) as cofactors for this covalent joining of DNA.
- DNA ligases;
- recombinant DNA;
- ligase chain reaction;
- mammalian ligases