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Thymidylate Synthesis

  1. Roy Kisliuk

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0003923



How to Cite

Kisliuk, R. 2006. Thymidylate Synthesis. eLS. .

Author Information

  1. Tufts University School of Medicine, Boston, Massachusetts, USA

Publication History

  1. Published Online: 27 JAN 2006

This is not the most recent version of the article. View current version (15 APR 2016)


Thymidylate, an essential component of DNA, is synthesized from deoxyuridylate and methylenetetrahydrofolate by thymidylate synthase, but dihydrofolate reductase and serine hydroxymethyltransferase are required to regenerate methylenetetrahydrofolate. Inhibitors of the synthase and the reductase arrest cell replication and are used in the treatment of cancer.


  • methylenetetrahydrofolate;
  • deoxyuridylate;
  • methotrexate;
  • fluorouracil;
  • Tomudex