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Restriction Enzymes

  1. Mala Mani,
  2. Karthikeyan Kandavelou,
  3. Joy Wu,
  4. Srinivasan Chandrasegaran

Published Online: 23 SEP 2005

DOI: 10.1038/npg.els.0005332



How to Cite

Mani, M., Kandavelou, K., Wu, J. and Chandrasegaran, S. 2005. Restriction Enzymes. eLS. .

Author Information

  1. Johns Hopkins University, Baltimore, Maryland, USA

Publication History

  1. Published Online: 23 SEP 2005


Restriction enzymes have played a key role in the completion of the initial sequencing and analysis of the first draft of the human genome. They were important tools in the cloning, analysis and sequencing of the various deoxyribonucleic acid fragments as well as in the construction of a physical map of the human genome.


  • restriction enzymes;
  • endonucleases;
  • modification enzymes;
  • methylases;
  • restriction–modification system;
  • restriction mapping;
  • recombinant DNA