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Capillary Electrophoresis

  1. Pier Giorgio Righetti

Published Online: 23 SEP 2005

DOI: 10.1038/npg.els.0005336

eLS

eLS

How to Cite

Righetti, P. G. 2005. Capillary Electrophoresis. eLS. .

Author Information

  1. University of Verona, Verona, Italy

Publication History

  1. Published Online: 23 SEP 2005

Abstract

Capillary zone electrophoresis is a fully automated approach to electrophoresis that uses narrow-bore (10–100 μm) fused-silica capillaries as a separation chamber and online detection of analytes. The increased heat dissipation allows the delivery of high field strengths (100–500 V cm−1). The use of minute sample amounts and volumes and the very fast analysis times make it an attractive technique in modern bioanalysis.

Keywords:

  • capillary electrophoresis;
  • temporal thermal gradients;
  • single-strand chain polymorphism;
  • isoelectric buffers