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Whole-genome Amplification

  1. Dagan Wells

Published Online: 23 SEP 2005

DOI: 10.1038/npg.els.0005341

eLS

eLS

How to Cite

Wells, D. 2005. Whole-genome Amplification. eLS. .

Author Information

  1. University College London, London, UK

Publication History

  1. Published Online: 23 SEP 2005

Abstract

Whole-genome amplification (WGA) techniques utilize the principles of the polymerase chain reaction (PCR) to copy enzymatically the deoxyribonucleic acid (DNA) contained within a sample. However, unlike most PCR applications, the amplification is nonspecific and theoretically provides an increase in the copy number of all genomic sequences. WGA is used in areas of research where minute DNA samples are routinely encountered. Applications include preimplantation genetic diagnosis, the creation of probes from microdissected or flow-sorted chromosomes, forensics and the study of ancient DNA samples.

Keywords:

  • DOP-PCR;
  • T-PCR;
  • alu-PCR;
  • linker adapter-PCR;
  • primer extension preamplification