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Restriction Mapping

  1. Burkhard Tümmler,
  2. Frauke Mekus

Published Online: 23 SEP 2005

DOI: 10.1038/npg.els.0005363



How to Cite

Tümmler, B. and Mekus, F. 2005. Restriction Mapping. eLS. .

Author Information

  1. Medizinische Hochschule Hannover, Hannover, Germany

Publication History

  1. Published Online: 23 SEP 2005


Deoxyribonucleic acid is partially or completely digested with one or more restriction endonucleases. The generated fragments are separated by size, and then ordered by combinatorial analysis to generate a physical map.


  • agarose gel electrophoresis;
  • physical map;
  • pulsed-field gel electrophoresis;
  • restriction endonuclease;
  • southern blot hybridization