Standard Article

Restriction Mapping

  1. Burkhard Tümmler,
  2. Frauke Mekus

Published Online: 23 SEP 2005

DOI: 10.1038/npg.els.0005363

eLS

eLS

How to Cite

Tümmler, B. and Mekus, F. 2005. Restriction Mapping. eLS. .

Author Information

  1. Medizinische Hochschule Hannover, Hannover, Germany

Publication History

  1. Published Online: 23 SEP 2005

Abstract

Deoxyribonucleic acid is partially or completely digested with one or more restriction endonucleases. The generated fragments are separated by size, and then ordered by combinatorial analysis to generate a physical map.

Keywords:

  • agarose gel electrophoresis;
  • physical map;
  • pulsed-field gel electrophoresis;
  • restriction endonuclease;
  • southern blot hybridization