Representational Difference Analysis
Published Online: 23 SEP 2005
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Groot, P. C. and van Oost, B. A. 2005. Representational Difference Analysis. eLS. .
- Published Online: 23 SEP 2005
Representational difference analysis (RDA) is a highly sensitive technique for the identification of differences between complex deoxyribonucleic acid samples. RDA is based on polymerase chain reaction amplification in combination with multiple rounds of subtractive hybridization and can be used for the identification of unknown pathogens, genetic lesions in cancer and polymorphic markers linked to a trait without the use of a preexisting map, as well as for the identification of differentially expressed genes.
- representational difference analysis;
- subtractive hybridization;
- kinetic enrichment;
- differential expression;
- polymerase chain reaction;