Standard Article

Primer Walking

  1. Eugene Sverdlov,
  2. Tatyana Azhikina

Published Online: 23 SEP 2005

DOI: 10.1038/npg.els.0005382

eLS

eLS

How to Cite

Sverdlov, E. and Azhikina, T. 2005. Primer Walking. eLS. .

Author Information

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

Publication History

  1. Published Online: 23 SEP 2005

Abstract

Primer walking is a step-by-step approach to sequencing long DNA templates from end to end that overcomes the inability of the Sanger chain termination method to read more than a few hundred bases in a single reaction. After an initial round of sequencing from a known sequence at one end of the template, each subsequent round is initiated from a new primer, which is based on the end of the sequence obtained from the previous reaction.

Keywords:

  • primer walking;
  • DNA sequencing;
  • genome;
  • gap closure;
  • hexamer library