Published Online: 23 SEP 2005
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Sverdlov, E. and Azhikina, T. 2005. Primer Walking. eLS. .
- Published Online: 23 SEP 2005
Primer walking is a step-by-step approach to sequencing long DNA templates from end to end that overcomes the inability of the Sanger chain termination method to read more than a few hundred bases in a single reaction. After an initial round of sequencing from a known sequence at one end of the template, each subsequent round is initiated from a new primer, which is based on the end of the sequence obtained from the previous reaction.
- primer walking;
- DNA sequencing;
- gap closure;
- hexamer library