Standard Article

Minigenes

  1. Laura E Warner,
  2. Jeffrey S Chamberlain

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0005684

eLS

eLS

How to Cite

Warner, L. E. and Chamberlain, J. S. 2006. Minigenes. eLS. .

Author Information

  1. University of Washington, Seattle, Washington, USA

Publication History

  1. Published Online: 27 JAN 2006

Abstract

A minigene is a compact version of a gene in which regions have been removed without affecting protein function. Understanding the structure and function of the protein produced from the gene of interest is critical in determining which portions of the gene can be deleted. The compact size of minigenes makes them easier to isolate and insert into cloning and viral vectors.

Keywords:

  • DMD;
  • dystrophin;
  • factor VIII;
  • hemophilia;
  • gene therapy