Standard Article

Protein Characterization: Analytical Approaches and Applications to Proteomics

  1. Kris Gevaert,
  2. Joël Vandekerckhove

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0005725

eLS

eLS

How to Cite

Gevaert, K. and Vandekerckhove, J. 2006. Protein Characterization: Analytical Approaches and Applications to Proteomics. eLS. .

Author Information

  1. Ghent University, Ghent, Belgium

Publication History

  1. Published Online: 27 JAN 2006

Abstract

Edman chemical sequencing and biological mass spectrometry are used routinely to identify and to characterize proteins. Two-dimensional polyacrylamide gel electrophoresis, highly sensitive mass spectrometry and sequence database searching algorithms have merged to form proteomics – the analysis of the protein complement of the genome.

Keywords:

  • mass spectrometry;
  • proteomics;
  • protein identification;
  • Edman degradation;
  • bioinformatics