Standard Article

Mass Spectrometry in Protein Characterization

  1. Hanno Steen1,
  2. Akhilesh Pandey2

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0005726



How to Cite

Steen, H. and Pandey, A. 2006. Mass Spectrometry in Protein Characterization. eLS. .

Author Information

  1. 1

    Harvard Medical School, Boston, Massachusetts, USA

  2. 2

    Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Publication History

  1. Published Online: 27 JAN 2006


The complete complement of proteins in a cell, the proteome, is highly complex because a single protein can exist in several different forms. Mass spectrometry has emerged as the tool of choice for protein identification as well as for characterization of posttranslational modifications.


  • mass spectrometry;
  • ionization;
  • posttranslational;
  • modifications;
  • phosphorylation;
  • quantification;