Standard Article

Cre–lox-Inducible Gene Targeting

  1. Brian Sauer

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0005979

eLS

eLS

How to Cite

Sauer, B. 2006. Cre–lox-Inducible Gene Targeting. eLS. .

Author Information

  1. Stowers Institute for Medical Research, Kansas City, Missouri, USA

Publication History

  1. Published Online: 27 JAN 2006

Abstract

Cre is a site-specific DNA recombinase that is useful for either targeted excision or activation of a desired gene in the genome in a temporally and spatially controlled manner. Cre-based recombination strategies also permit the design of precise chromosome translocations, deletions and duplications, which facilitate the genetic manipulation of the eukaryotic genome.

Keywords:

  • transgenic mice;
  • chromosome engineering;
  • DNA recombinase;
  • conditional mutation;
  • embryonic stem cells