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Two-dimensional Gel Electrophoresis

  1. Shao-En Ong1,
  2. Cynthia Mui Yee Rosa Liang2

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0006191

eLS

eLS

How to Cite

Ong, S.-E. and Rosa Liang, C. M. Y. 2006. Two-dimensional Gel Electrophoresis. eLS. .

Author Information

  1. 1

    University of Southern Denmark, Odense, Denmark

  2. 2

    National University of Singapore, Singapore, Malaysia

Publication History

  1. Published Online: 27 JAN 2006

Abstract

Two-dimensional gel electrophoresis separates proteins according to their isoelectric points and molecular weight. Recent developments allowing improved resolution, detection and reproducibility, coupled with sensitive protein identification by mass spectrometry has made this methodology one of the most common tools for the study of proteomes in the postgenomic age.

Keywords:

  • quantitation;
  • proteomics;
  • mass spectrometry;
  • protein separation;
  • two-dimensional gel