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Preparation Artefacts in Proteomics: Technological Issues

  1. Pier Giorgio Righetti,
  2. Mahmoud Hamdan

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0006192

eLS

eLS

How to Cite

Righetti, P. G. and Hamdan, M. 2006. Preparation Artefacts in Proteomics: Technological Issues. eLS. .

Author Information

  1. University of Verona, Verona, Italy

Publication History

  1. Published Online: 27 JAN 2006

Abstract

In addition to taking into account the more than 200 endogenously produced posttranslational modifications, a detailed analysis of proteins separated by two-dimensional electrophoresis must consider other modifications that a protein can experience during the various steps of its separation.

Keywords:

  • proteomics;
  • protein alkylation;
  • iodoacetamide;
  • acrylamide;
  • 4-vinylpyridine;
  • electrophoresis