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Proteomics: A Shotgun Approach without Two-dimensional Gels

  1. Claire Delahunty,
  2. John R Yates III

Published Online: 27 JAN 2006

DOI: 10.1038/npg.els.0006197

eLS

eLS

How to Cite

Delahunty, C. and Yates, J. R. 2006. Proteomics: A Shotgun Approach without Two-dimensional Gels. eLS. .

Author Information

  1. The Scripps Research Institute, La Jolla, California, USA

Publication History

  1. Published Online: 27 JAN 2006

This is not the most recent version of the article. View current version (15 OCT 2014)

Abstract

Proteome analysis has been most commonly accomplished by the combination of two-dimensional electrophoresis and mass spectrometry, but this method suffers some drawbacks that limit its usefulness for large-scale analysis. New ‘shotgun’ techniques allow for the detection of proteins from complex mixtures and are rapidly replacing gel-based methods for certain applications.

Keywords:

  • proteomics;
  • mass spectrometry;
  • tandem mass spectrometry;
  • two-dimensional gels;
  • shotgun proteomics;
  • multidimensional protein identification technique;
  • microcapillary liquid chromatography;
  • matrix assisted laser desorption/ionization