Proteomics: A Shotgun Approach without Two-dimensional Gels
Published Online: 27 JAN 2006
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Delahunty, C. and Yates, J. R. 2006. Proteomics: A Shotgun Approach without Two-dimensional Gels. eLS. .
- Published Online: 27 JAN 2006
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Proteome analysis has been most commonly accomplished by the combination of two-dimensional electrophoresis and mass spectrometry, but this method suffers some drawbacks that limit its usefulness for large-scale analysis. New ‘shotgun’ techniques allow for the detection of proteins from complex mixtures and are rapidly replacing gel-based methods for certain applications.
- mass spectrometry;
- tandem mass spectrometry;
- two-dimensional gels;
- shotgun proteomics;
- multidimensional protein identification technique;
- microcapillary liquid chromatography;
- matrix assisted laser desorption/ionization