These authors contributed equally.
Effect of Voluntary Exercise on Genetically Obese Cpefat/fat Mice: Quantitative Proteomics of Serum
Article first published online: 6 SEP 2012
2004 North American Association for the Study of Obesity (NAASO)
Volume 12, Issue 7, pages 1179–1188, July 2004
How to Cite
Yuan, Q., Fontenele-Neto, J. D. and Fricker, L. D. (2004), Effect of Voluntary Exercise on Genetically Obese Cpefat/fat Mice: Quantitative Proteomics of Serum. Obesity Research, 12: 1179–1188. doi: 10.1038/oby.2004.147
The costs of publication of this article were defrayed, in part, by the payment of page charges. This article must, therefore, be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Issue published online: 6 SEP 2012
- Article first published online: 6 SEP 2012
- Received for review January 12, 2004; Accepted in final form May 24, 2004
- peptide processing;
- carboxypeptidase E;
Objective: To compare the effect of voluntary exercise on body weight, food consumption, and levels of serum proteins between wild-type and carboxypeptidase E-deficient (Cpefat/fat) mice.
Research Methods and Procedures: Study 1 consisted of three groups of female mice: Cpefat/fat mice with continuous access to exercise wheels for 3 weeks (n = 4); wild-type C57BKS mice with access to exercise wheels for 3 weeks (n = 4); and sedentary Cpefat/fat mice (n = 3). Activity, body weight, and food consumption were monitored for this period and a subsequent 9-week period without exercise wheels. Study 2 consisted of four groups of male mice (n = 6 to 7 each): Cpefat/fat mice with exercise wheels, wild-type mice with exercise wheels, and Cpefat/fat and wild-type mice without exercise wheels. Body weight and food consumption were measured over 4 weeks. Sera were collected, and the protein profile was determined by 2-dimensional gel electrophoresis and mass spectrometry.
Results: Cpefat/fat mice were moderately hyperphagic but lost weight during the initial exercise period because of greater energy expenditure. The effect of exercise was temporary, and the mice gained weight after the second week. Several serum proteins were found to be altered by exercise: haptoglobin was decreased by exercise in Cpefat/fat mice, and several kallikreins were increased by exercise in wild-type mice.
Discussion: The access to exercise wheels provided an initial weight loss in Cpefat/fat mice, but this effect was offset by elevated food consumption. The serum proteomics results indicated that Cpefat/fat and wild-type mice differed in their response to exercise.