A Survey of Genes Differentially Expressed in Subcutaneous and Visceral Adipose Tissue in Men

Authors

  • Marie-Claude Vohl,

    Corresponding author
    1. Lipid Research Center and Laval Hospital Research Center, School of Medicine, Laval University, Québec, Canada
    2. Department of Food Science and Nutrition and Laval Hospital Research Center, School of Medicine, Laval University, Québec, Canada
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  • Rob Sladek,

    1. McGill University and Genome Québec Innovation Centre, Montreal, Québec, Canada
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  • Julie Robitaille,

    1. Lipid Research Center and Laval Hospital Research Center, School of Medicine, Laval University, Québec, Canada
    2. Department of Food Science and Nutrition and Laval Hospital Research Center, School of Medicine, Laval University, Québec, Canada
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  • Scott Gurd,

    1. McGill University and Genome Québec Innovation Centre, Montreal, Québec, Canada
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  • Picard Marceau,

    1. Department of Surgery, Laval Hospital, Québec City, Québec, Canada
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  • Denis Richard,

    1. Department of Anatomy and Physiology and Laval Hospital Research Center, School of Medicine, Laval University, Québec, Canada
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  • Thomas J. Hudson,

    1. McGill University and Genome Québec Innovation Centre, Montreal, Québec, Canada
    2. Department of Human Genetics, McGill University, Montréal, Canada
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  • André Tchernof

    1. Department of Food Science and Nutrition and Laval Hospital Research Center, School of Medicine, Laval University, Québec, Canada
    2. Molecular Endocrinology and Oncology Laboratory, Québec, Canada
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  • The online version of this article (available at http:www.obesityresearch.org) contains supplemental tables.

    According to U.S. code, all journals requesting payment of author page charges in order to defray the cost of publication are required to publish a disclaimer. This article must, therefore, be marked “advertisement” in compliance with U.S.C. Section 1734 solely to indicate this fact.

Lipid Research Center, 2705 Laurier Blvd. (TR-93), Quebec City, PQ, Canada G1V 4G2. E-mail: Marie-Claude.Vohl@crchul.ulaval.ca

Abstract

Adipose tissue located within the abdominal cavity has been suggested to be functionally and metabolically distinct from that of the subcutaneous compartment. These differences could play a role in obesity-related complications. The aim of this study was to compare gene expression profiles of subcutaneous and visceral adipose tissues of 10 nondiabetic, normolipidemic obese men. Affymetrix human U133A arrays (10 arrays for subcutaneous fat samples and 10 arrays for visceral fat samples) were used. Differential gene expression was confirmed by real-time polymerase chain reaction in a subset of genes. A total of 5894 transcripts were detected in both depots in all 10 subjects, and 409 transcripts representing 347 encoded genes were differentially expressed. Of these, 131 genes were expressed at higher levels in subcutaneous adipose tissue, and 216 were expressed more abundantly in visceral fat. Differentially expressed profiles included genes of the Wnt signaling pathway, as well as CEPBA and HOX genes. In addition, genes involved in lipolytic stimuli and cytokine secretion were differentially expressed. The identification of a consistent and rather uniform pattern of differentially expressed genes between the two fat depots using multiple array replicates (10 arrays per fat compartment) generated new perspectives for future research on regional differences in adipose tissue biology.

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