The online version of this article (available at http:www.obesityresearch.org) contains supplemental appendices.
Lack of Association between Human G-Protein β3 Subunit Variant and Overweight in Japanese Workers*
Article first published online: 6 SEP 2012
2004 North American Association for the Study of Obesity (NAASO)
Volume 12, Issue 1, pages 4–8, January 2004
How to Cite
Suwazono, Y., Okubo, Y., Kobayashi, E., Miura, K., Morikawa, Y., Ishizaki, M., Kido, T., Nakagawa, H. and Nogawa, K. (2004), Lack of Association between Human G-Protein β3 Subunit Variant and Overweight in Japanese Workers. Obesity Research, 12: 4–8. doi: 10.1038/oby.2004.2
- Issue published online: 6 SEP 2012
- Article first published online: 6 SEP 2012
- Received for review July 31, 2003; Accepted in final form November 17, 2003
- G proteins;
- population studies;
- multiple logistic regression analysis
We examined the association of the G-protein β3 subunit gene (GNB3) C825T polymorphism with overweight in Japanese workers. This cross-sectional study used multivariate analysis to investigate whether a polymorphism in the C825T polymorphism was associated independently with overweight when factors such as age and lifestyle were taken into account. The study in 1453 men and 1172 women involved identifying subjects with the C825T genotype using the polymerase chain reaction, followed by restriction fragment-length polymorphism analysis. Overweight was defined as a BMI ≥25 kg/m2. Genotype distributions for C825T in overweight men (CC = 80, CT = 162, TT = 80) and women (CC = 52, CT = 91, TT = 40) were not significantly different from normal-weight men (CC = 278, CT = 588, TT = 265) and women (CC = 242, CT = 549, TT = 198). The allele distributions were also not significantly different between either sex. The power of the study was estimated as 98% in men and 81% in women based on the allelic frequencies reported in a previous positive study in Chinese subjects. Multiple logistic regression analysis showed that the genotype was not significantly associated with overweight. In conclusion, this study indicated that the GNB3 C825T polymorphism is not a significant factor for overweight in Japanese people.