Evaluation of Reference Genes for Studies of Gene Expression in Human Adipose Tissue

Authors

  • Britt G. Gabrielsson,

    Corresponding author
    1. Research Center for Endocrinology and Metabolism, Department of Internal Medicine, the Sahlgrenska Academy, Göteborg, Sweden
      Research Center for Endocrinology and Metabolism, Gröna Stråket 8, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. E-mail: britt.gabrielsson@medic.gu.se
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  • Louise E. Olofsson,

    1. Research Center for Endocrinology and Metabolism, Department of Internal Medicine, the Sahlgrenska Academy, Göteborg, Sweden
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  • Anders Sjögren,

    1. Department of Mathematical Statistics, Chalmers University of Technology, Göteborg, Sweden
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  • Margareta Jernås,

    1. Research Center for Endocrinology and Metabolism, Department of Internal Medicine, the Sahlgrenska Academy, Göteborg, Sweden
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  • Anna Elander,

    1. Department of Plastic Surgery, the Sahlgrenska Academy, Göteborg, Sweden
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  • Malin Lönn,

    1. Research Center for Endocrinology and Metabolism, Department of Internal Medicine, the Sahlgrenska Academy, Göteborg, Sweden
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  • Mats Rudemo,

    1. Department of Mathematical Statistics, Chalmers University of Technology, Göteborg, Sweden
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  • Lena M. S. Carlsson

    1. Research Center for Endocrinology and Metabolism, Department of Internal Medicine, the Sahlgrenska Academy, Göteborg, Sweden
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  • The costs of publication of this article were defrayed, in part, by the payment of page charges. This article must, therefore, be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Research Center for Endocrinology and Metabolism, Gröna Stråket 8, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden. E-mail: britt.gabrielsson@medic.gu.se

Abstract

Objective: The aim of this study was to evaluate reference genes for expression studies of human adipose tissue.

Research Methods and Procedures: Using 52 human adipose tissue expression profiles (HU95), 10 putative reference genes with the lowest variation in expression levels were selected for further studies. Expression stability of these 10 novel and 5 previously established reference genes was evaluated by real-time reverse transcriptase-polymerase chain reaction analysis. For this purpose, 44 adipose tissue biopsies from 27 subjects were chosen to include a wide range of parameters such as sex, age, BMI, depot origin, biopsy procedure, and effects of nutrition.

Results: LRP10 was identified as the gene with the least variation in expression levels. The frequently used reference genes RPLP0, 18S rRNA, PPIA, ACTB, and GAPD were ranked as 4, 6, 7, 8, and 10, respectively.

Discussion: Our results suggest that LRP10 is a better choice as reference for expression studies of human adipose tissue compared with the most frequently used reference genes.

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