Adipocyte Enhancer-Binding Protein 1 Modulates Adiposity and Energy Homeostasis
Article first published online: 6 SEP 2012
2007 North American Association for the Study of Obesity (NAASO)
Volume 15, Issue 2, pages 288–302, February 2007
How to Cite
Ro, H.-S., Zhang, L., Majdalawieh, A., Kim, S.-W., Wu, X., Lyons, P. J., Webber, C., Ma, H., Reidy, S. P., Boudreau, A., Miller, J. R., Mitchell, P. and McLeod, R. S. (2007), Adipocyte Enhancer-Binding Protein 1 Modulates Adiposity and Energy Homeostasis. Obesity, 15: 288–302. doi: 10.1038/oby.2007.569
- Issue published online: 6 SEP 2012
- Article first published online: 6 SEP 2012
- Received for review May 08, 2006, Accepted in final from September 07, 2006
- adipose tissue;
- energy balance;
- mouse models;
- phosphatase and tensin homolog deleted on chromosome ten;
- mitogen-activated protein kinase
Objective: To determine whether adipocyte enhancer binding protein (AEBP) 1, a transcriptional repressor that is down-regulated during adipogenesis, functions as a critical regulator of adipose tissue homeostasis through modulation of phosphatase and tensin homolog deleted on chromosome ten (PTEN) tumor suppressor activity and mitogen-activated protein kinase (MAPK) activation.
Research Methods and Procedures: We examined whether AEBP1 physically interacts with PTEN in 3T3-L1 cells by coimmunoprecipitation analysis. We generated AEBP1-null mice and examined the physiological role of AEBP1 as a key modulator of in vivo adiposity. Using adipose tissue from wild-type and AEBP1-null animals, we examined whether AEBP1 affects PTEN protein level.
Results: AEBP1 interacts with PTEN, and deficiency of AEBP1 increases adipose tissue PTEN mass. AEBP1-null mice have reduced adipose tissue mass and enhanced apoptosis with suppressed survival signal. Primary pre-adipocytes from AEBP1-null adipose tissues exhibit lower basal MAPK activity with defective proliferative potential. AEBP1-null mice are also resistant to diet-induced obesity, suggesting a regulatory role for AEBP1 in energy homeostasis.
Discussion: Our results suggest that AEBP1 negatively regulates adipose tissue PTEN levels, in conjunction with its role in proliferation and differentiation of pre-adipocytes, as a key functional role in modulation of in vivo adiposity.