Effects of Pronounced Weight Loss on Adiponectin Oligomer Composition and Metabolic Parameters

Authors

  • Julia Engl,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
    2. These authors contributed equally to this work.
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  • Thomas Bobbert,

    1. Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
    2. Department of Endocrinology, Diabetes and Nutrition, Charité-Universitätsmedizin Berlin, Berlin, Germany
    3. These authors contributed equally to this work.
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  • Christian Ciardi,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
    2. These authors contributed equally to this work.
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  • Markus Laimer,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
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  • Tobias Tatarczyk,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
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  • Susanne Kaser,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
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  • Helmut Weiss,

    1. Department of Surgery, Medical University Innsbruck, Innsbruck, Austria
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  • Clemens Molnar,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
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  • Herbert Tilg,

    1. Department of Medicine, Academic Teaching Hospital, Hall, Austria
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  • Josef R. Patsch,

    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
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  • Joachim Spranger,

    1. Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany
    2. Department of Endocrinology, Diabetes and Nutrition, Charité-Universitätsmedizin Berlin, Berlin, Germany
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  • Christoph F. Ebenbichler

    Corresponding author
    1. Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Innsbruck, Austria
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Clinical Department of Internal Medicine, Clinical Division of General Internal Medicine, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria. E-mail: Christoph.Ebenbichler@uibk.ac.at

Abstract

Objective: Adiponectin is an adipocytokine secreted into circulation in three isoforms. The aim of the study was to investigate changes of adiponectin isoforms during profound weight loss and its relation to anthropomorphometric and metabolic parameters.

Research Methods and Procedures: Thirteen severely obese female subjects were examined before and 1 year after surgical treatment. Total adiponectin was determined by radioimmunosorbent assay, and oligomer composition was detected by nondenaturing Western blot.

Results: BMI decreased substantially (p < 0.001), which was associated with an increase of total adiponectin from 12.9 ± 5.9 to 14.3 ± 6.1 μg/mL (p = 0.055). Medium molecular weight (MMW) adiponectin increased from 7.5 ± 3.6 to 9.1 ± 4.1 μg/mL (p = 0.009), whereas high (HMW) and low molecular weight adiponectin remained unchanged. Δ values of total adiponectin correlated significantly with Δ values of anthropometric parameters. Similar correlations were found for Δ values of MMW (Δ weight: r2 = 0.4132, p = 0.0178; Δ BMI: r2 = 0.3319, p = 0.0393; Δ fat mass: r2 = 0.5202, p = 0.0054).

Discussion: Thus, profound weight loss was associated with an increase in total adiponectin, which was mainly and consistently caused by increases in MMW adiponectin (p = 0.009). These changes result in a shift from low molecular weight to MMW and HMW adiponectin isoforms, which may be related to improvements in both anthropometric and metabolic parameters.

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