LMNA mRNA Expression Is Altered in Human Obesity and Type 2 Diabetes

Authors

  • Merce Miranda,

    Corresponding author
    1. Endocrinology and Diabetes Unit, Research Department, University Hospital of Tarragona Joan XXIII, “Pere Virgili” Institute, “Rovira i Virgili” University, Tarragona, Spain
    2. CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain
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  • Matilde R. Chacón,

    1. Endocrinology and Diabetes Unit, Research Department, University Hospital of Tarragona Joan XXIII, “Pere Virgili” Institute, “Rovira i Virgili” University, Tarragona, Spain
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  • Cristina Gutiérrez,

    1. Endocrinology and Diabetes Unit, Research Department, University Hospital of Tarragona Joan XXIII, “Pere Virgili” Institute, “Rovira i Virgili” University, Tarragona, Spain
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  • Nuria Vilarrasa,

    1. Endocrinology and Diabetes Unit, University Hospital of Bellvitge, L'Hospitalet de Llobregat, Spain
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  • Jose M. Gómez,

    1. Endocrinology and Diabetes Unit, University Hospital of Bellvitge, L'Hospitalet de Llobregat, Spain
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  • Enric Caubet,

    1. Surgery Department, “St. Pau i Sta. Tecla” Hospital, Tarragona, Spain
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  • Ana Megía,

    1. Endocrinology and Diabetes Unit, Research Department, University Hospital of Tarragona Joan XXIII, “Pere Virgili” Institute, “Rovira i Virgili” University, Tarragona, Spain
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  • Joan Vendrell

    1. Endocrinology and Diabetes Unit, Research Department, University Hospital of Tarragona Joan XXIII, “Pere Virgili” Institute, “Rovira i Virgili” University, Tarragona, Spain
    2. CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain
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(mmiranda.hj23.ics@gencat.net)

Abstract

Objective: The aim of this study was to analyze lamin A/C mRNA levels in abdominal subcutaneous adipose tissue in two conditions—obesity and type 2 diabetes—that share common inflammatory and metabolic features, and to assess their relationship with selected inflammatory and adipogenic genes.

Methods and Procedures: This is a cross-sectional study involving 52 nondiabetic and 54 type 2 diabetes patients. Anthropometrical and analytical measurements (glycemic, lipidic, and inflammatory profiles) were performed, and mRNA expression was determined using real-time PCR.

Results: Lamin A and C isoforms are expressed differentially. Lamin A/C mRNA levels were increased in obese and in type 2 diabetes patients. We also observed a strong relationship between both isoforms (B = 2.218, P < 0.001) and among lamin C mRNA expression and adipogenic (sterol-responsive element binding protein-1 (SREBP1c)) and inflammatory (interleukin-6 (IL-6)) markers (B = 0.854, P = 0.001, and B = 0.557, P < 0.001, respectively).

Discussion: These data suggest that lamin A/C may be involved in the adipocyte gene profile observed in obesity and type 2 diabetes.

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