The first two authors contributed equally to this work.
Rac1 Is a Possible Link Between Obesity and Oxidative Stress in Chinese Overweight Adolescents
Article first published online: 31 DEC 2012
2012 North American Association for the Study of Obesity (NAASO)
Volume 20, Issue 11, pages 2233–2240, November 2012
How to Cite
Sun, M., Huang, X., Yan, Y., Chen, J., Wang, Z., Xie, M. and Li, J. (2012), Rac1 Is a Possible Link Between Obesity and Oxidative Stress in Chinese Overweight Adolescents. Obesity, 20: 2233–2240. doi: 10.1038/oby.2012.63
- Issue published online: 31 DEC 2012
- Article first published online: 31 DEC 2012
- Received 17 August 2011; accepted 06 March 2012; advance online publication 29 March 2012
Enhanced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity in the monocytes occurred in metabolic syndrome, hypertension, diabetes and obese patients in adults. However, whether NADPH oxidase is involved in the oxidative stress of overweight adolescents without comorbidities is still unclear. This study aimed to identify whether and how NADPH oxidase plays a crucial role in overweight adolescents. The study was performed in 93 overweight adolescents and 31 normal weight controls. Moreover, 87 overweight adolescents were enrolled in weight-loss program. Demographics characteristics, anthropometrics, composition and clinical characteristics were analyzed. Oxidative stress indexes including the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in plasma and the expression of NADPH oxidase in the monocytes were examined. Overweight adolescents showed a higher oxidative stress state, as indicated by decreased SOD activity and elevated MDA level (P < 0.01). Furthermore, increased NADPH oxidase activity in the monocytes was accompanied by Rac1 upregulation. A significant positive bivariate correlation was found between Rac1 expression and MDA (r = 0.289). There also was a significant positive bivariate correlation between Rac1 expression and obesity-related indexes including BMI (r = 0.227) and percentage of trunk fat (r = 0.233). Data from weight-loss program reinforced the results. Partial correlation analysis indicated that obesity-induced oxidative stress and Rac1 expression is a consequence of aberrant glucose-lipid metabolism in overweight adolescents. In conclusion, we provided novel data showing that NADPH oxidase in the monocytes was highly activated by enhancing Rac1 expression in Chinese overweight adolescents and Rac1 may act as a link between obesity and oxidative stress in overweight adolescents.