Effect of vanilloid drugs on gastrointestinal transit in mice
Version of Record online: 29 JAN 2009
2001 British Pharmacological Society
British Journal of Pharmacology
Volume 132, Issue 7, pages 1411–1416, April 2001
How to Cite
Izzo, A. A., Capasso, R., Pinto, L., Carlo, G. D., Mascolo, N. and Capasso, F. (2001), Effect of vanilloid drugs on gastrointestinal transit in mice. British Journal of Pharmacology, 132: 1411–1416. doi: 10.1038/sj.bjp.0703975
- Issue online: 29 JAN 2009
- Version of Record online: 29 JAN 2009
- (Received May 9, 2000, Revised December 18, 2000, Accepted January 22, 2001)
- primary afferent neurones;
- vanilloid receptors;
- cannabinoid receptors;
- intestinal motility
We have studied the effect of capsaicin, piperine and anandamide, drugs which activate vanilloid receptors and capsazepine, a vanilloid receptor antagonist, on upper gastrointestinal motility in mice.
Piperine (0.5 – 20 mg kg−1 i.p.) and anandamide (0.5 – 20 mg kg−1 i.p.), dose-dependently delayed gastrointestinal motility, while capsaicin (up to 3 mg kg−1 i.p.) was without effect. Capsazepine (15 mg kg−1 i.p.) neither per se affected gastrointestinal motility nor did it counteract the inhibitory effect of both piperine (10 mg kg−1) and anandamide (10 mg kg−1).
A per se non effective dose of SR141716A (0.3 mg kg−1 i.p.), a cannabinoid CB1 receptor antagonist, counteracted the inhibitory effect of anandamide (10 mg kg−1) but not of piperine (10 mg kg−1). By contrast, the inhibitory effect of piperine (10 mg kg−1) but not of anandamide (10 mg kg−1) was strongly attenuated in capsaicin (75 mg kg−1 in total, s.c.)-treated mice.
Pretreatment of mice with NG-nitro-L-arginine methyl ester (25 mg kg−1 i.p.), yohimbine (1 mg kg−1, i.p.), naloxone (2 mg kg−1 i.p.), or hexamethonium (1 mg kg−1 i.p.) did not modify the inhibitory effect of both piperine (10 mg kg−1) and anandamide (10 mg kg−1).
The present study indicates that the vanilloid ligands anandamide and piperine, but not capsaicin, can reduce upper gastrointestinal motility. The effect of piperine involves capsaicin-sensitive neurones, but not vanilloid receptors, while the effect of anandamide involves cannabinoid CB1, but not vanilloid receptors.
British Journal of Pharmacology (2001) 132, 1411–1416; doi:10.1038/sj.bjp.0703975