The protective effect of resveratrols on ischaemia-reperfusion injuries of rat hearts is correlated with antioxidant efficacy

Authors


Department of Physiology, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-San, Taoyuan, Taiwan. E-mail: jkc508@mail.cgu.edu.tw

Abstract

  • Dietary antioxidants are thought to be beneficial in reducing the incidence of coronary heart disease. In this study, we compared resveratrol and analogues on their antioxidation and free radical scavenging activities to their protective effects on ischaemia-reperfusion induced injuries of rat hearts.

  • Astringinin (3,3′,4′,5-tetrahydroxystilbene) was shown to be a more potent inhibitor than other analogues against Cu2+-induced LDL (low-density lipoprotein) oxidation, as measured by the formation of conjugated diene and TBARS (thiobarbituric acid-reactive substance) and by the electrophoretic mobility of the oxidized LDL.

  • Resveratrol (trans-3,4′,5-trihydroxystilbene) and astringinin scavenged the stable free radical DPPH (1,1-diphenyl-2-picryl-hydrazyl) with an IC0.200 of 7.1 and 4.3 μM, respectively.

  • Astringinin has a superoxide anion scavenging activity about 160 fold more potent than resveratrol.

  • After a 30 min global ischemia followed by 2 h reperfusion, astringinin (10 μM) significantly reduced infarct size, superoxide anion production and increased functional recovery of the coronary flow in Langendorff-perfused rat hearts.

  • The result showed there is a positive correlation between the anti-oxidation and cardioprotective activities among these phenolic compounds. Our finding together with the fact that astringinin is more water-soluble than resveratrol suggest that astringinin could potentially be used as an anti-oxidant and cardioprotective agent in biological systems.

British Journal of Pharmacology (2002) 135, 1627–1633; doi:10.1038/sj.bjp.0704637

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