Contribution of vanilloid receptors to the overt nociception induced by B2 kinin receptor activation in mice
Article first published online: 30 JAN 2009
2004 British Pharmacological Society
British Journal of Pharmacology
Volume 141, Issue 5, pages 787–794, March 2004
How to Cite
Ferreira, J., Da Silva, G. L. and Calixto, J. B. (2004), Contribution of vanilloid receptors to the overt nociception induced by B2 kinin receptor activation in mice. British Journal of Pharmacology, 141: 787–794. doi: 10.1038/sj.bjp.0705546
- Issue published online: 30 JAN 2009
- Article first published online: 30 JAN 2009
- (Received July 14, 2003, Revised August 19, 2003, Accepted September 17, 2003)
- B2 receptor;
- vanilloid receptor;
The vanilloid receptor (TRPV1) is viewed as a molecular integrator of several nociceptive stimuli. In the present study, we have investigated the role played by TRPV1 in the nociceptive response induced by the peripheral activation of kinin B2 receptor in mice.
The intraplantar (i.pl.) administration of bradykinin (BK) and the selective B2 agonist Tyr8-BK, or the vanilloid agonists resiniferatoxin and capsaicin, into the mouse paw induced a dose-related overt nociception of short duration. The B2 receptor antagonist Hoe 140 inhibited BK-induced, but not capsaicin-induced, nociceptive response. On the other hand, the TRPV1 antagonist capsazepine inhibited both capsaicin- and BK-mediated nociception.
Repeated injections of BK or capsaicin produced desensitization to their nociceptive response. Capsaicin desensitization greatly reduced BK-induced nociception, but in contrast, the desensitization to BK increased the capsaicin response.
Administration of low doses of capsaicin or acidified saline did not produce nociception when administered alone, but caused a pronounced effect when administered in association with a subthreshold dose of BK. Moreover, the degeneration of the subset of primary afferent fibers, sensitive to capsaicin, abolished both capsaicin- and BK-induced nociception.
The inhibition of phospholipase C (PLC), protein kinase C or phospholipase A2 markedly decreased the nociception caused by BK, but not that of capsaicin. BK administration increased leukotriene B4 levels in the injected paw. Likewise, BK-induced overt nociception was decreased by lipoxygenase (LOX) inhibition.
These results demonstrate that BK produces overt nociception mediated by TRPV1 receptor stimulation, via PLC pathway activation and LOX product formation.
British Journal of Pharmacology (2004) 141, 787–794. doi:10.1038/sj.bjp.0705546