Hydrogen sulphide is a mediator of carrageenan-induced hindpaw oedema in the rat

Authors

  • Madhav Bhatia,

    Corresponding author
    1. Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore
      Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore. E-mail: mbhatia@nus.edu.sg
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  • Jenab Sidhapuriwala,

    1. Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore
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  • Shabbir M Moochhala,

    1. Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore
    2. Centre for Biomedical Sciences, Defence Medical and Environmental Research Institute, Defence Science Organization, Singapore 117510, Singapore
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  • Philip K Moore

    1. Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore
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Department of Pharmacology, Cardiovascular Biology Research Group, National University of Singapore, 18 Medical Drive, Singapore 117597, Singapore. E-mail: mbhatia@nus.edu.sg

Abstract

Hydrogen sulphide (H2S) is a naturally occurring gas, with potent vasodilator activity. In this report, we identify a role for H2S in carrageenan-induced hindpaw oedema in the rat. Intraplantar injection of carrageenan (150 μl, 2% (w v−1)) resulted in an increase in hindpaw H2S synthesising enzyme activity and increased myeloperoxidase (MPO) activity. Pretreatment (i.p. 60 min before carrageenan) with DL-propargylglycine (PAG, 25–75 mg kg−1), an inhibitor of the H2S synthesising enzyme cystathionine-γ-lyase (CSE), significantly reduced carrageenan-induced hindpaw oedema in a dose-dependent manner (e.g. increase in hindpaw weight at 3 h, saline: 0.12±0.017 g; carrageenan, 1.39±0.037 g; PAG, 50 mg kg−1, 1.11±0.06 g, n=10) and MPO activity (fold increase) in the hindpaw (saline: 1.0±0.12; carrageenan, 2.92±0.45 g; PAG, 50 mg kg−1, 1.1±0.22, n=10); PAG (50 mg kg−1) also inhibited H2S synthesising enzyme activity (nmol μg DNA−1) in the hindpaw in a dose-dependent manner (saline, 0.46±0.05; carrageenan, 0.71±0.08 g; PAG, 50 mg kg−1, 0.17±0.05, n=10).

British Journal of Pharmacology (2005) 145, 141–144. doi:10.1038/sj.bjp.0706186

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