Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretzi
Version of Record online: 3 JAN 2012
2009 Société Française des Microscopies and Société Biologie Cellulaire de France
Biology of the Cell
Volume 101, Issue 11, pages 645–659, November 2009
How to Cite
Niwano, T., Takatori, N., Kumano, G. and Nishida, H. (2009), Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretzi. Biology of the Cell, 101: 645–659. doi: 10.1042/BC20090042
- Issue online: 3 JAN 2012
- Version of Record online: 3 JAN 2012
- Received 5 June 2009; Accepted 8 June 2009
- convergent extension;
- Dishevelled (Dsh);
- Wnt/planar cell polarity (PCP) pathway
Background information. In the embryos of various animals, the body elongates after gastrulation by morphogenetic movements involving convergent extension. The Wnt/PCP (planar cell polarity) pathway plays roles in this process, particularly mediolateral polarization and intercalation of the embryonic cells. In ascidians, several factors in this pathway, including Wnt5, have been identified and found to be involved in the intercalation process of notochord cells.
Results. In the present study, the role of the Wnt5 genes, Hr-Wnt5α (Halocynthia roretzi Wnt5α) and Hr-Wnt5β, in convergent extension was investigated in the ascidian H. roretzi by injecting antisense oligonucleotides and mRNAs into single precursor blastomeres of various tissues, including notochord, at the 64-cell stage. Hr-Wnt5α is expressed in developing notochord and was essential for notochord morphogenesis. Precise quantitative control of its expression level was crucial for proper cell intercalation. Overexpression of Wnt5 proteins in notochord and other tissues that surround the notochord indicated that Wnt5α plays a role within the notochord, and is unlikely to be the source of polarizing cues arising outside the notochord. Detailed mosaic analysis of the behaviour of individual notochord cells overexpressing Wnt5α indicated that a Wnt5α-manipulated cell does not affect the behaviour of neighbouring notochord cells, suggesting that Wnt5α works in a cell-autonomous manner. This is further supported by comparison of the results of Wnt5α and Dsh (Dishevelled) knockdown experiments. In addition, our results suggest that the Wnt/PCP pathway is also involved in mediolateral intercalation of cells of the ventral row of the nerve cord (floor plate) and the endodermal strand.
Conclusion. The present study highlights the role of the Wnt5α signal in notochord convergent extension movements in ascidian embryos. Our results raise the novel possibility that Wnt5α functions in a cell-autonomous manner in activation of the Wnt/PCP pathway to polarize the protrusive activity that drives convergent extension.