1 Biyan Lu and Zhizhong Ye contributed equally to this work.
MEK/ERK pathway mediates cytoprotection of salvianolic acid B against oxidative stress-induced apoptosis in rat bone marrow stem cells
Article first published online: 2 JAN 2013
2010 International Federation for Cell Biology
Cell Biology International
Volume 34, Issue 11, pages 1063–1068, November 2010
How to Cite
Lu, B., Ye, Z., Deng, Y., Wu, H. and Feng, J. (2010), MEK/ERK pathway mediates cytoprotection of salvianolic acid B against oxidative stress-induced apoptosis in rat bone marrow stem cells. Cell Biology International, 34: 1063–1068. doi: 10.1042/CBI20090126
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 12 September 2009/ 27 January 2010; accepted 15 July 2010
- bone marrow stem cell;
- oxidative stress;
- salvianolic acid B
To improve the survival and/or differentiation of grafted BMSCs (bone marrow stem cells) represents one of the challenges for the promising cell-based therapy. Considerable reports have implicated Sal B (salvianolic acid B), a potent aqueous extract of Salvia miltiorrhiza, in enhancing the survival of cells under various conditions. In this study, we investigated the effect of Sal B on H2O2-induced apoptosis in rat BMSCs, focusing on the survival signalling pathways. Results indicated that the MEK [MAPK (mitogen-activated protein kinase)/ERK (extracellular-signal-regulated kinase) kinase] inhibitor (PD98059) and 10 μM Sal B remarkably prevented BMSCs from H2O2-induced apoptosis through attenuating caspase-3 activation, which is accompanied by the significant up-regulation of Bcl-2. In addition, the ROS (reactive oxygen species) accumulation was also reduced after Sal B treatment. Furthermore, Sal B inhibited the ERK1/2 phosphorylations stimulated by H2O2. Taken together, our results showed that H2O2-induced apoptosis in BMSCs via the ROS/MEK/ERK1/2 pathway and Sal B may exert its cytoprotection through mediating the pathway.