These authors contributed equally to this work.
Involvement of ethylene and nitric oxide in cell death in mastoparan-treated unicellular alga Chlamydomonas reinhardtii
Version of Record online: 2 JAN 2013
2010 International Federation for Cell Biology
Cell Biology International
Volume 34, Issue 3, pages 301–308, March 2010
How to Cite
Yordanova, Z. P., Iakimova, E. T., Cristescu, S. M., Harren, F. J., Kapchina-Toteva, V. M. and Woltering, E. J. (2010), Involvement of ethylene and nitric oxide in cell death in mastoparan-treated unicellular alga Chlamydomonas reinhardtii. Cell Biology International, 34: 301–308. doi: 10.1042/CBI20090138
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- Received 26 March 2009/ 17 September 2009; accepted 9 November 2009
- cell death;
- Chlamydomonas reinhardtii;
- nitric oxide
This work demonstrates a contribution of ethylene and NO (nitric oxide) in MP (mastoparan)-induced cell death in the green algae Chlamydomonas reinhardtii. Following MP treatment, C. reinhardtii showed massive cell death, expressing morphological features of PCD (programmed cell death). A pharmacological approach involving combined treatments with MP and ethylene- and NO-interacting compounds indicated the requirement of trace amounts of both ethylene and NO in MP-induced cell death. By employing a carbon dioxide laser-based photoacoustic detector to measure ethylene and a QCL (quantum cascade laser)-based spectrometer for NO detection, simultaneous increases in the production of both ethylene and NO were observed following MP application. Our results show a tight regulation of the levels of both signalling molecules in which ethylene stimulates NO production and NO stimulates ethylene production. This suggests that, in conjunction with the elicitor, NO and ethylene cooperate and act synchronously in the mediation of MP-induced PCD in C. reinhardtii. To the best of our knowledge, this is the first report on the functional significance of ethylene and NO in MP-induced cell death.