Recombinant PBD-1 (porcine beta-defensin 1) expressed in the milk by transplanting transgenic mES-like-derived cells into mouse mammary gland

Authors

  • Hai-Jun Huang,

    1. Key Laboratory of Swine Breeding and Genetics, Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Huazhong Agricultural University, Ministry of Education, Wuhan 430070, Peoples Republic of China
    2. Department of Animal Biotechnology and Cell Engineering, Wuhan Academy of Agricultural Science, Wuhan 430065, Peoples Republic of China
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  • Qi-Shuang Gao,

    1. Department of Animal Biotechnology and Cell Engineering, Wuhan Academy of Agricultural Science, Wuhan 430065, Peoples Republic of China
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  • Yun-Guo Qian,

    1. Department of Animal Biotechnology and Cell Engineering, Wuhan Academy of Agricultural Science, Wuhan 430065, Peoples Republic of China
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  • Yu-Dan Zhang,

    1. Department of Animal Nutrition, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, Peoples Republic of China
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  • Bi-Fei Tao,

    1. Department of Animal Biotechnology and Cell Engineering, Wuhan Academy of Agricultural Science, Wuhan 430065, Peoples Republic of China
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  • Min Xiang,

    1. Department of Animal Biotechnology and Cell Engineering, Wuhan Academy of Agricultural Science, Wuhan 430065, Peoples Republic of China
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  • Jian Peng,

    1. Histological and Embryological Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Peoples Republic of China
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  • Si-Wen Jiang,

    Corresponding author
    1. Key Laboratory of Swine Breeding and Genetics, Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction Huazhong Agricultural University, Ministry of Education, Wuhan 430070, Peoples Republic of China
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  • Ben Hause║

    1. Department of Cell Engineering, Newport Laboratories, 1524 Prairie Drive, Worthington, MN 56187, U.S.A.
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Abstract

ES (embryonic stem)-derived cells have been investigated in many animal models of severe injury and degenerative disease. However, few studies have examined the ability of ES-derived cells to improve functional outcome following partially damaged breast and also the modification of mammary tissue to produce costly proteins. This study investigates the feasibility of implanting mES-dK (mouse ES-derived keratinocytes-like) cells stably transfected with a mammary gland special expression vector for the PBD-1 (porcine beta-defensin 1) in developing mammary glands. Our aim was to assess the ability of cell grafting to improve functional outcome following partial damage of the breast, also on the breast modification mammary tissue in mice for the production of PBD-1 protein secreted in the milk. Our results showed that the ratios of the surviving cells labelled with the myoepithelial or luminal cell markers, EMA (epithelial membrane antigen) and CALLA, were 41.7±15.2% and 28.4±9.6%, respectively, which revealed that transplanted mES-dK cells survived, integrated in vivo and differentiated into myoepithelial or luminal cells. In addition, Western blot analysis showed that 37.5% (3 out of 8) female transplanted mice had PBD-1 expression in their milk and reached 0.4998, 0.5229 and 0.5195 μg/ml, respectively.

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