These authors contributed equally to this work.
Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice
Article first published online: 2 JAN 2013
2010 International Federation for Cell Biology
Cell Biology International
Volume 34, Issue 8, pages 791–797, August 2010
How to Cite
Xu, J., Wang, F., Tang, Z., Zhan, Y., Zhang, J., Yan, Q., Xiong, Y., Xie, X., Wu, J. and Zhang, S. (2010), Role of leukaemia inhibitory factor in the induction of pluripotent stem cells in mice. Cell Biology International, 34: 791–797. doi: 10.1042/CBI20090484
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 19 December 2009/ 2 April 2010; accepted 14 April 2010
- induced pluripotent stem (iPS) cell;
- leukaemia inhibitory factor (LIF);
- nuclear reprogramming
iPS (induced pluripotent stem) cells can be induced from somatic cells in mice by genetic manipulation. Most previously established mouse iPS cell lines have been derived using feeder layers supplemented with exogenous LIF (leukaemia inhibitory factor). Although a feeder-free induction system has been developed in recent studies, LIF is still required for reprogramming, but its role in the generation of mouse iPS cells has remained elusive. In this study, we investigated its contribution to the induction of pluripotency. Our results showed that LIF activates AP (alkaline phosphatase) through a c-Myc-dependent mechanism. Moreover, it acts as a protective factor during the transition from AP-positive colonies to Oct3/4-positive cells. These findings illustrate a mechanism by which LIF may integrate signalling into reprogramming.