Stem cell-conditioned medium does not protect against kidney failure

Authors

  • Yousof Gheisari,

    Corresponding author
    1. Department of Stem Cell Biology and Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
      Yousof Gheisari and Naser Ahmadbeigi contributed equally to this work.
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  • Naser Ahmadbeigi,

    Corresponding author
    1. Department of Stem Cell Biology and Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
    2. SABZ Biomedicals, Tehran, Iran
      Yousof Gheisari and Naser Ahmadbeigi contributed equally to this work.
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  • Mahmood Naderi,

    1. Department of Stem Cell Biology and Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
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  • Seyed Mahdi Nassiri,

    1. Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
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  • Samad Nadri,

    1. Department of Stem Cell Biology and Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran, Iran
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  • Masoud Soleimani

    Corresponding author
    1. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
      To whom correspondence should be addressed (email soleim_m@modares.ac.ir).
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Yousof Gheisari and Naser Ahmadbeigi contributed equally to this work.

To whom correspondence should be addressed (email soleim_m@modares.ac.ir).

Abstract

Paracrine secretion of mediators may be the main route by which stem cells protect against injuries. Stem cells commonly secrete different bioactive molecules. In this study, we examined the hypothesis that administration of conditioned media of stem cells can diminish the burden of kidney injury. A mouse model of cisplatin-induced nephropathy was developed to test the putative renoprotective effects of conditioned media of human umbilical cord blood USSCs (unrestricted somatic stem cells) as well as mouse bone marrow MSCs (mesenchymal stem cells). None of these two types of conditioned medium could protect against kidney failure in terms of serum urea and creatinine, histopathologic examinations and physical activity score. Neither MSC- nor USSC-conditioned media were effective in protecting against kidney injury in our study. Possible explanations for our observations are offered, and related literature is reviewed.

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