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Sp1 plays an important role in regulating the transcription of ZNF313

Authors

  • Xiaolin Liao,

    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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  • Wenqian Deng,

    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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  • Yilu Lu,

    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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  • Dachang Tao,

    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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  • Yunqiang Liu,

    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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  • Sizhong Zhang,

    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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  • Yongxin Ma

    Corresponding author
    1. Department of Medical Genetics, State Key Labotatory of Biotherapy, West China Hospital, Sichuan University, Renmin Nanlu, Section317, Chengdu 610041, Peoples Republic of China
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email Mayongxin@gmail.com

Abstract

The ZNF313 gene has the highest transcription level in fertile male testes and may be related to human spermatogenesis. The deletion-mutated plasmids of ZNF313 promoter were constructed and transfected into HEK293 cells. The result showed that the fragment from nt −157 to +8 has a basal transcriptional activity. A functional Sp1 binding site was identified by site-directed mutation test and mithramycin A treatment. A 447-bp based at +233 to −213 exhibits a characteristic CpG island, which overlaps with the promoter region. Our work suggests that ZNF313 is controlled at the transcriptional level, and a common mechanism controlling the basal transcription of ZNF313 gene exists.

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