Promotion of human adipose-derived stem cell proliferation mediated by exogenous nucleosides

Authors

  • Fernando Rodríguez-Serrano,

    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Pablo Álvarez,

    1. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Octavio Caba,

    1. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
    2. Department of Health Science, University of Jan, 23071 Jan, Spain
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  • Manuel Picón,

    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Juan A. Marchal,

    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Macarena Perán,

    1. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
    2. Department of Health Science, University of Jan, 23071 Jan, Spain
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  • José Prados,

    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Consolación Melguizo,

    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Ana R. Rama,

    1. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
    2. Department of Health Science, University of Jan, 23071 Jan, Spain
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  • Houria Boulaiz,

    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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  • Antonia Aránega

    Corresponding author
    1. Department of Human Anatomy and Embryology, University of Granada, 18012 Granada, Spain
    2. Biopathology and Regenerative Medicine Institute IBIMER, 18100 Granada, Spain
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email aranega@ugr.es

Abstract

Adult stem cells are becoming the best option for regenerative medicine because they have low tumourigenic potential and permit autologous transplantation, even without in vitro culture. Our objectives were to evaluate the effects of exogenous nucleosides on the proliferation of hASCs (human adipose-derived stem cells), with or without co-treatment with 5-aza (5-azacytidine), and to analyse the expression of lamin A/C during cardiomyocyte differentiation of these cells. We isolated hASCs from human lipoaspirates that were positive for mesenchymal stem cell markers. We found that 5-aza induces a dose-dependent inhibition of hASC proliferation [IC50 (inhibitory concentration 50): 5.37 μM], whereas exogenous nucleosides significantly promote the proliferation of hASCs and partially revert the antiproliferative effect of the drug. Multipotentiality of isolated hASCs was confirmed by adipogenic, osteogenic and cardiomyogenic induction. 5-Aza-induced cells expressed cardiac troponins I and T and myosin light chain 2, myocardial markers that were directly correlated with lamin A/C expression. Our results support the importance of the nucleoside supplementation of media to improve conditions for the expansion and maintenance of hASCs in culture. In addition, the quantification of lamin A/C expression appears to be a good marker for the characterization of cardiomyocyte differentiation of stem cells that has rarely been used.

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