Gelatin induces trophectoderm differentiation of mouse embryonic stem cells

Authors

  • Sha Peng,

    Corresponding author
    1. Shaanxi Stem Cell Engineering and Technology Research Center, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
    2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
    3. College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
      To whom correspondence should be addressed (email pengsha123@yahoo.com.cn or hwang101@163.com).
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  • Jinlian Hua,

    1. Shaanxi Stem Cell Engineering and Technology Research Center, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
    2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
    3. College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
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  • Xuanhong Cao,

    1. College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
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  • Huayan Wang

    Corresponding author
    1. Shaanxi Stem Cell Engineering and Technology Research Center, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
    2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
    3. College of Veterinary Medicine, Northwest AF University, Yangling, Shaanxi 712100, Peoples Republic of China
      To whom correspondence should be addressed (email pengsha123@yahoo.com.cn or hwang101@163.com).
    Search for more papers by this author

To whom correspondence should be addressed (email pengsha123@yahoo.com.cn or hwang101@163.com).

Abstract

In this study, we selected gelatin as ECM (extracellular matrix) to support differentiation of mES (mouse embryonic stem) cells into TE (trophectoderm), as gelatin was less expensive and widely used. We found that 0.2% and 1.5% gelatin were the suitable concentrations to induce TE differentiation by means of detecting Cdx2 expression using real-time PCR. Moreover, about 15% cells were positive for Cdx2 staining after 6 days differentiation. We discovered that the expressions of specific markers for TE, such as Cdx2, Eomes, Hand1 and Esx1 were prominently increased after gelatin induction. Meanwhile, the expression of Oct4 was significantly decreased. We also found that inhibition of the BMP (bone morphogenetic protein) signalling by Noggin could promote mES cells differentiation into TE, whereas inhibition of the Wnt signalling by Dkk1 had the contrary effect. This could be used as a tool to study the differentiation and function of early trophoblasts as well as further elucidating the molecular mechanism during abnormal placental development.

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