Characterization of bone-marrow-derived rat mesenchymal stem cells depending on donor age

Authors

  • Kamila Gala,

    1. Department of Immunology, Transplantology and Internal Disease, Transplantation Institute, Medical University of Warsaw, Nowogrodzka 59, 02006 Warsaw, Poland
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  • Anna Burdzińska,

    1. Department of Immunology, Transplantology and Internal Disease, Transplantation Institute, Medical University of Warsaw, Nowogrodzka 59, 02006 Warsaw, Poland
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  • Marta Idziak,

    1. Department of Immunology, Transplantology and Internal Disease, Transplantation Institute, Medical University of Warsaw, Nowogrodzka 59, 02006 Warsaw, Poland
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  • Jolanta Makula,

    1. Department of Immunology, Transplantology and Internal Disease, Transplantation Institute, Medical University of Warsaw, Nowogrodzka 59, 02006 Warsaw, Poland
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  • Leszek Pa̧czek

    Corresponding author
    1. Department of Immunology, Transplantology and Internal Disease, Transplantation Institute, Medical University of Warsaw, Nowogrodzka 59, 02006 Warsaw, Poland
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To whom correspondence should be addressed (email leszek.paczek@wum.edu.pl).

Abstract

It is generally accepted that autologous transfers, as non-immunogenic, constitute the safest approach in cellular transplantations. However, this attitude is often associated with the need for isolation and extracorporeal propagation of cells derived from aged patients. Thus the knowledge about relationship between aging and the properties of MSCs (mesenchymal stem cells) is crucial in developing new clinical strategies. The aim of this study was to perform complex comparison of MSC derived from young and aged individuals, which included phenotype, proliferating rate, osteogenic and adipogenic potential and secretory activity. Evaluated populations were isolated from bone marrow of 3-month-old and 24-month-old rats. There was no significant difference in membrane antigen expression and PDT (population doubling time). Additionally, the adipogenic and osteogenic potential did not vary between studied populations. The reaction of MSCs to either mitogen [bFGF (basic fibroblast growth factor)] or oxidative stress (H2O2) in vitro displayed a very similar pattern in both analysed populations. There was no difference in TGFβ1 (transforming growth factor β1) and VEGF (vascular endothelial growth factor) secretion measured by ELISA test and gene expression evaluated by real-time PCR. However, the expression of the gene for IL-1α (interleukin-1α) was 8-fold lower in oMSC (MSC isolated from old rats). These results indicate that aging individuals can be considered as candidates for autologous transplantation of bone-marrow-derived MSCs.

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