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Apoptosis by aloe-emodin is mediated through down-regulation of calpain-2 and ubiquitin-protein ligase E3A in human hepatoma Huh-7 cells

Authors

  • Won Jeon,

    1. Department of Biological Science, Gachon University of Medicine and Science, 5342 Yeonsudong, Yeonsugu, Incheon 406799, Republic of Korea
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  • Young Keul Jeon,

    1. Department of Biological Science, Gachon University of Medicine and Science, 5342 Yeonsudong, Yeonsugu, Incheon 406799, Republic of Korea
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  • Myeong Jin Nam

    Corresponding author
    1. Department of Biological Science, Gachon University of Medicine and Science, 5342 Yeonsudong, Yeonsugu, Incheon 406799, Republic of Korea
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To whom correspondence should be addressed (email genetx@hanmail.net).

Abstract

Natural flavonoids are associated with anti-proliferation of cancer growth. However, the antioxidant and anti-proliferation effects of AE (aloe-emodin) have not been well studied. We have investigated how AE affects the proliferation of hepatic hepatocellular carcinoma cells and exerts an anti-cancer effect. The cytotoxic effect of AE was demonstrated using an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay and Huh-7 cells were inhibited by AE treatment in both dose- and time-dependent manners. The IC50 level of AE was ∼75 μM. AE also has anti-proliferative effects via induction of DNA damage and apoptosis. 2-DE (two-dimensional electrophoresis) revealed that several proteins were related to the anti-cancer effects of AE. CAPN2 (calpain-2) and UBE3A (ubiquitin-protein ligase E3A), which are associated with the apoptosis signalling pathway, were verified by Western blotting. AE exhibited potent anti-proliferative effects on Huh-7 cells via down-regulation of CAPN2 and UBE3A. The findings support the possibility of AE being a chemopreventative agent.

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