Genes expression profiling of peripheral blood cells of patients with hepatocellular carcinoma

Authors

  • Peng-jun Zhang,

    Corresponding author
    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. Medical College, Nankai University, Tianjin, People's Republic of China
    3. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Run Wei,

    Corresponding author
    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Xin-yu Wen,

    Corresponding author
    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Liang Ping,

    1. Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Cheng-bin Wang,

    1. Department of Clinical Laboratory, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Zhen-nan Dong,

    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Xin-xin Deng,

    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Wang Bo,

    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. Medical College, Nankai University, Tianjin, People's Republic of China
    3. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Chen Bin,

    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. Medical College, Nankai University, Tianjin, People's Republic of China
    3. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Ya-ping Tian

    Corresponding author
    1. Department of Clinical Biochemistry, Chinese PLA General Hospital, Beijing, People's Republic of China
    2. Medical College, Nankai University, Tianjin, People's Republic of China
    3. State Key Laboratory of Kidney Disease, Chinese PLA General Hospital, Beijing, People's Republic of China
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These authors have contributed equally to this work.

To whom correspondence should be addressed (email tianyp61@gmail.com).

Abstract

HCC (hepatocellular carcinoma) is often diagnosed at an advanced stage with poor prognosis. Peripheral blood may be useful in cancer classification, and therefore we investigated the gene expression found by Affymetrix HG-U133 Plus2.0 microarray, with samples from nine HCC patients and five healthy NC (normal controls). A total of 726 probe sets showed significant differences based on the criteria of P<0.05 and absolute fold change >2. The genes were related to many biological functions, including immune response, transcription regulation and metabolism processes. Ten genes [IL-8 (interleukin 8), GOS2 (G0/G1 switch gene 2), CXCR4 (CXC chemokine receptor 4), FOS, RPS24 (40S ribosomal protein S24), HAP90AA1, PFDN5, RPL27, GZMA and PFN1] showing significant differences were confirmed by real-time PCR in 54 HCC patients and 56 healthy NC. Seven genes [IL-8, GOS2, CXCR4, FOS, RPS24, HSP90AA1 (heat shock protein 90AA1) and PFN1] showed significant difference both in RT—PCR (reverse transcription—PCR) and microarray. Expression of IL-8 and FOS proteins was up-regulated in HCC compared with healthy controls. A gene signature in peripheral blood which can distinguish HCC patients and healthy controls may have been identified.

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