ZNF580, a novel C2H2 zinc-finger transcription factor, interacts with the TGF-β signal molecule Smad2

Authors

  • Yuyu Luo,

    1. Graduate School of Tianjin Medical University, Tianjin 300070, Peoples Republic of China
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  • Wenliang Hu,

    1. Department of Physiology and Pathophysiology, Medical College of Chinese Peoples Armed Police Force, Tianjin 300162, Peoples Republic of China
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  • Ruicheng Xu,

    1. Department of Physiology and Pathophysiology, Medical College of Chinese Peoples Armed Police Force, Tianjin 300162, Peoples Republic of China
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  • Bing Hou,

    1. Department of Physiology and Pathophysiology, Medical College of Chinese Peoples Armed Police Force, Tianjin 300162, Peoples Republic of China
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  • Lijun Zhang,

    1. Graduate School of Tianjin Medical University, Tianjin 300070, Peoples Republic of China
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  • Wencheng Zhang

    Corresponding author
    1. Graduate School of Tianjin Medical University, Tianjin 300070, Peoples Republic of China
    2. Department of Physiology and Pathophysiology, Medical College of Chinese Peoples Armed Police Force, Tianjin 300162, Peoples Republic of China
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To whom correspondence should be addressed (email wenchengzhang@yahoo.com).

Abstract

ZNF580 (gene ID 51157), a novel gene encoding a C2H2 (Cys2-His2) zinc-finger transcription factor, may be involved in the maintenance of vascular endothelium homoeostasis. To investigate the physiological role of the transcription factor ZNF580, we screened human foetal brain cDNA library with a yeast two-hybrid system and identified 14 proteins that interact with ZNF580. The interaction between ZNF580 and Smad2 was confirmed by co-immunoprecipitation. Co-localization between endogenous ZNF580 and Smad2 was mainly found in the nuclei of EA.hy926 endothelial cells with immunofluorescence and confocal microscopy. Our results suggest that ZNF580 is a binding partner of Smad2 and is involved in the signal transduction of the TGF-β (transforming growth factor-β) signalling pathway, which provides a basis for additional research to investigate the role of ZNF580 in the maintenance of vascular endothelium homoeostasis and the onset of atherosclerotic diseases.

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