Myostatin regulates preadipocyte differentiation and lipid metabolism of adipocyte via ERK1/2

Authors

  • Fengna Li,

    1. Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, Peoples Republic of China
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  • Huansheng Yang,

    1. Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, Peoples Republic of China
    2. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, Peoples Republic of China
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  • Yehui Duan,

    1. Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, Peoples Republic of China
    2. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, Peoples Republic of China
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  • Yulong Yin

    Corresponding author
    1. Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, Peoples Republic of China
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To whom correspondence should be addressed (email yinyulong@isa.ac.cn).

Abstract

Myostatin is known as an inhibitor of muscle development, but its role in adipogenesis and lipid metabolism is still unclear, especially the underlying mechanisms. Here, we demonstrated that myostatin inhibited 3T3-L1 preadipocyte differentiation into adipocyte by suppressing C/EBPα (CCAAT/enhancer-binding protein α) and PPARα (peroxisome-proliferator-activated receptor α), also activated ERK1/2 (extracellular-signal-regulated kinase 1/2). Furthermore, myostatin enhanced the phosphorylation of HSL (hormone-sensitive lipase) and ACC (acetyl-CoA carboxylase) in fully differentiated adipocytes, as well as ERK1/2. Besides, we noted that myostatin markedly raised the levels of leptin and adiponectin release and mRNA expression during preadipocyte differentiation, but the levels were inhibited by myostatin treatments in fully differentiated adipocytes. These results suggested that myostatin suppressed 3T3-L1 preadipocyte differentiation and regulated lipid metabolism of mature adipocyte, in part, via activation of ERK1/2 signalling pathway.

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