All authors contributed equally to this work.
Multipotent differentiation of the EGFP gene transgenic stem cells derived from amniotic fluid of goat at terminal gestational age
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2011 International Federation for Cell Biology
Cell Biology International
Volume 35, Issue 12, pages 1243–1246, December 2011
How to Cite
Zheng, Y.-M., Zheng, Y.-L., He, X.-Y., He, X.-N., Zhao, X. and Sai, W.-J.-F. (2011), Multipotent differentiation of the EGFP gene transgenic stem cells derived from amniotic fluid of goat at terminal gestational age. Cell Biology International, 35: 1243–1246. doi: 10.1042/CBI20110128
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 24 February 2011/19 April 2011; accepted 24 May 2011
- amniotic fluid-derived stem cells;
- EGFP gene;
We have isolated stem cells from amniotic fluid of goat at terminal gestational age and transferred the EGFP (enhanced green fluorescent protein) gene into the stem cells previously. The aim of this study was to determine whether the transgenic stem cells have the capability of multipotent differentiation. The transgenic stem cells were induced to differentiate into neurogenic, adipogenic, osteogenic and endothelial cells in vitro. Markers associated with AFS (amniotic fluid-derived stem) cells and the differentiated cells were tested by RT—PCR (reverse transcription—PCR). The results demonstrated that the transgenic AFS cells were capable of self-renewal, a defining property of stem cells. AFS cells were positive for the undifferentiated cell markers, Oct4, Nanog, Sox2 and Hes1, while following differentiation cells expressed markers for neurogenic cells such as astrocyte [GFAP (glial fibrillary acidic protein)] and NSE (neuron-specific enolase), adipogenic cells [LPL+ (lipoprotein lipase+)], osteogenic cells (osteocalcin+ and osteonectin+) and endothelium [CD34+ and eNOS+ (endothelial nitric oxide synthase)]. The results demonstrated that the EGFP gene transgenic AFS cells have the capability of multipotent differentiation, which means that the transgenic AFS cells may be useful in cell-transplantation studies in future.