Quantity and proliferation rate of mesenchymal stem cells in human cord blood during gestation

Authors

  • Lan-Lan Ma,

    1. Key Laboratory of Cell Biology of China Ministry of Public Health, Department of Stem Cells and Regenerative Medicine, China Medical University, Shen Yang, Peoples Republic of China
    2. Physiology Center, China Medical University, Shen Yang, Peoples Republic of China
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  • Fan-Biao Meng,

    1. Stem Cell and Regeneration Program, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, Peoples Republic of China
    2. Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, Peoples Republic of China
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  • Ping Shi,

    1. Key Laboratory of Cell Biology of China Ministry of Public Health, Department of Stem Cells and Regenerative Medicine, China Medical University, Shen Yang, Peoples Republic of China
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  • Gang Li,

    Corresponding author
    1. Stem Cell and Regeneration Program, Li Ka Shing Institute of Health Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, Peoples Republic of China
    2. Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, Peoples Republic of China
    3. MOE Key Laboratory of Regenerative Medicine, School of Biomedical Sciences, Basic Medical Sciences Building, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, Peoples Republic of China
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  • Xi-Ning Pang

    Corresponding author
    1. Key Laboratory of Cell Biology of China Ministry of Public Health, Department of Stem Cells and Regenerative Medicine, China Medical University, Shen Yang, Peoples Republic of China
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Correspondence may be addressed to either of these authors (email mkf@xanum.uam.mx) or gangli@ort.cuhk.edu.hk

Abstract

UCB (umbilical cord blood) as a resource of MSCs (mesenchymal stem cells) is widely accepted, but the quantity and characteristics of UCB-MSCs from different gestational ages have not been well studied. We have quantified the number of MSCs in UCB at different gestational ages using a multi-colour flowcytometer and compared the cell proliferation rates of these UCB-MSCs. Defining MSCs as CD44+/CD105+/CD34 −/CD45 population, their numbers declined in the UCB at the gestational age. Proliferation rates were significantly higher in UCB before term than at full term. Non-full term UCB samples may be a better source of MSCs.

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