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PDGFRβ triggered by bFGF promotes the proliferation and migration of endothelial progenitor cells via p-ERK signalling

Authors

  • Sufen Guo,

    Corresponding author
    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
      These authors equally contributed to this work.
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  • Libo Yu,

    Corresponding author
    1. Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China
      These authors equally contributed to this work.
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  • Yongxia Cheng,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Caijuan Li,

    1. Department of Ultrasound, Hongqi Hospital of Mudanjiang Medical University, Mudanjiang, People's Republic of China
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  • Jifei Zhang,

    1. Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
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  • Jindan An,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Hongwei Wang,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Bin Yan,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Tao Zhan,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Yong Cao,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Huizhe Zheng,

    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
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  • Zhiqiang Li

    Corresponding author
    1. Department of Pathology, Mudanjiang Medical University, Mudanjiang, People's Republic of China
    2. Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province, Mudanjiang, People's Republic of China
      To whom correspondence should be addressed (email sfguo9630@yahoo.com.cn).
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These authors equally contributed to this work.

To whom correspondence should be addressed (email sfguo9630@yahoo.com.cn).

Abstract

We have examined the effects of bFGF (basic fibroblast growth factor) on p-ERK (phosphorylated extracellular signal-regulated kinase) through PDGFRβ (platelet-derived growth factor receptor β) in the proliferation and migration of EPCs (endothelial progenitor cells). EPC migration was detected using the Transwell system. The expression of PDGFRβ mRNA and protein, total ERK and p-ERK proteins was respectively assessed by real-time PCR and Western blottings. bFGF promote the proliferation and migration of EPCs, the effects of bFGF being implemented by activating ERK signalling through the expression of PDGFRβ, whereas an anti-bFGF antibody and inhibitor of PDGF (platelet-derived growth factor) receptor kinase (AG1296) could respectively decrease the expression of PDGFRβ mRNA and protein and p-ERK protein. Total ERK protein did not change under the same experimental conditions, and an inhibitor of p-ERK (PD98059) inhibited the proliferation and migration of EPCs. The findings strongly suggest that a PDGFRβ/p-ERK signalling pathway triggered by bFGF plays an important role in the proliferation and migration of EPCs.

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