Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells

Authors

  • Yan-Hui Cen,

    1. Center Laboratory of School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Wen-Wen Guo,

    1. Center Laboratory of School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Bin Luo,

    1. Center Laboratory of School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Yong-Da Lin,

    1. Center Laboratory of School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
    2. School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Qing-Mei Zhang,

    1. Center Laboratory of School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Su-Fang Zhou,

    1. School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Guo-Rong Luo,

    1. School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Shao-Wen Xiao,

    1. Department of Neurosurgery, 1st Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
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  • Xiao-Xun Xie

    Corresponding author
    1. Center Laboratory of School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
    2. School of Preclinical Medicine, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, People's Republic of China
      To whom correspondence should be addressed (email xiaoxunxie@gmail.com).
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To whom correspondence should be addressed (email xiaoxunxie@gmail.com).

Abstract

OY-TES-1 is a member of the CTA (cancer-testis antigen) group expressed in a variety of cancer and restrictedly expressed in adult normal tissues, except for testis. To determine whether MSCs (mesenchymal stem cells) express OY-TES-1 and its possible roles on MSCs, OY-TES-1 expression in MSCs isolated from human bone marrow was tested with RT (reverse transcription)-PCR, immunocytochemistry and Western blot. Using RNAi (RNA interference) technology, OY-TES-1 expression was knocked down followed by analysing cell viability, cell cycle, apoptosis and migration ability. MSCs expressed OY-TES-1 at both mRNA and protein levels. The down-regulation of OY-TES-1 expression in these MSCs caused cell growth inhibition, cell cycle arrest, apoptosis induction and migration ability attenuation. Through these primary results it was suggested that OY-TES-1 may influence the biological behaviour of MSCs.

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