You have full text access to this OnlineOpen article

Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic β-amylase from Calystegia sepium (hedge bindweed) rhizomes

  1. Els J. M. Van Damme1,
  2. Jialiang Hu1,
  3. Annick Barre2,
  4. Bettina Hause3,
  5. Geert Baggerman4,
  6. Pierre Rougé2,
  7. Willy J. Peumans1

Article first published online: 20 DEC 2001

DOI: 10.1046/j.0014-2956.2001.02584.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 268, Issue 23, pages 6263–6273, December 2001

Additional Information

How to Cite

Van Damme, E. J. M., Hu, J., Barre, A., Hause, B., Baggerman, G., Rougé, P. and Peumans, W. J. (2001), Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic β-amylase from Calystegia sepium (hedge bindweed) rhizomes. European Journal of Biochemistry, 268: 6263–6273. doi: 10.1046/j.0014-2956.2001.02584.x

Author Information

  1. 1

    Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Leuven, Belgium;

  2. 2

    Institut de Pharmacologie et Biologie Structurale, Unité Mixte de Recherche Centre National de la Recherche Scientifique 5089, Toulouse, France;

  3. 3

    Institute of Plant Biochemistry, Halle, Germany;

  4. 4

    Laboratory of Developmental Physiology and Molecular Biology, Katholieke Universiteit Leuven, Leuven, Belgium

* E. J. M. Van Damme, Katholieke Universiteit Leuven, Laboratory for Phytopathology and Plant Protection, Willem de Croylaan 42, 3001 Leuven, Belgium. Fax: + 32 16 322976, Tel.: + 32 16 322379, E-mail: Els.VanDamme@agr.kuleuven.ac.be

Publication History

  1. Issue published online: 20 DEC 2001
  2. Article first published online: 20 DEC 2001
  3. (Received 6 July 2001, revised 5 October 2001, accepted 8 October 2001)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (2145K)

Keywords:

  • β-amylase;
  • Calystegia sepium;
  • hedge bindweed;
  • immunolocalization;
  • vegetative storage protein

An abundant catalytically active β-amylase (EC 3.2.1.2) was isolated from resting rhizomes of hedge bindweed (Calystegia sepium). Biochemical analysis of the purified protein, molecular modeling, and cloning of the corresponding gene indicated that this enzyme resembles previously characterized plant β-amylases with regard to its amino-acid sequence, molecular structure and catalytic activities. Immunolocalization demonstrated that the β-amylase is exclusively located in the cytoplasm. It is suggested that the hedge bindweed rhizome β-amylase is a cytoplasmic vegetative storage protein.

View Full Article (HTML) Get PDF (2145K)