• Please log in or register to access this feature.
You have full text access to this OnlineOpen article

Molecular cloning, bacterial expression and properties of Rab31and Rab32

New blood platelet Rab proteins

  1. Xiankun Bao1,
  2. Andrea E. Faris2,
  3. Elliott K. Jang1,
  4. Richard J. Haslam1,2

Article first published online: 30 OCT 2003

DOI: 10.1046/j.0014-2956.2001.02645.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 269, Issue 1, pages 259–271, January 2002

Additional Information

How to Cite

Bao, X., Faris, A. E., Jang, E. K. and Haslam, R. J. (2002), Molecular cloning, bacterial expression and properties of Rab31and Rab32. European Journal of Biochemistry, 269: 259–271. doi: 10.1046/j.0014-2956.2001.02645.x

Author Information

  1. 1

    Departments of Pathology and Molecular Medicine and

  2. 2

    Biochemistry, McMaster University, Hamilton, Ontario, Canada

* R. J. Haslam, Department of Pathology and Molecular Medicine, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5. Fax: + 905 777 7856, Tel.: + 905 525 9140 Ext. 22475, E-mail: haslamr@mcmaster.ca

  1. Enzyme: glutathione S-transferase (EC 2.5.1.18).

Publication History

  1. Issue published online: 30 OCT 2003
  2. Article first published online: 30 OCT 2003
  3. (Received 10 July 2001, revised 16 October 2001, accepted 30 October 2001)

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (778K)

References

  • 1
    Zerial, M. & McBride, H. (2001) Rab proteins as membrane organizers. Nat. Rev. 2, 107–117.
  • 2
    Siess, W. (1989) Molecular mechanisms of platelet activation. Physiol. Rev. 69, 58–178.
  • 3
    Karniguian, A., Zahraoui, A. & Tavitian, A. (1993) Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins. Proc. Natl Acad. Sci. USA 90, 7647–7651.
  • 4
    Shirakawa, R., Yoshioka, A., Horiuchi, H., Nishioka, H., Tabuchi, A. & Kita, T. (2000) Small GTPase Rab4 regulates Ca2+-induced α-granule secretion in platelets. J. Biol. Chem. 275, 33844–33849.
  • 5
    Nagata, K., Okano, Y., Suzuki, T. & Nozawa, Y. (1993) Evidence for the presence of a low Mr GTP-binding protein, ram p25, in human platelet membranes. Platelets 4, 268–274.
  • 6
    Chen, D., Guo, J., Miki, T., Tachibana, M. & Gahl, W.A. (1997) Molecular cloning and characterization of Rab27a and Rab27b, novel human Rab proteins shared by melanocytes and platelets. Biochem. Mol. Med. 60, 27–37.
  • 7
    Nagata, K. & Nozawa, Y. (1993) Subcellular localization of a low Mr GTP-binding protein, c25KG, in resting and stimulated human platelets. Biochem. Biophys. Res. Commun. 195, 1081–1088.
  • 8
    Pereira-Leal, J.B. & Seabra, M.C. (2000) The mammalian Rab family of small GTPases: definition of family and subfamily sequence motifs suggests a mechanism for functional specificity in the Ras superfamily. J. Mol. Biol. 301, 1077–1087.DOI: 10.1006/jmbi.2000.4010
  • 9
    Bao, X., Jang, E.K. & Haslam, R.J. (1996) Molecular cloning of two novel Rab proteins from human platelets. FASEB J. 10, A1011.
  • 10
    Bao, X., Faris, A.E. & Haslam, R.J. (1997) Cloning, tissue distribution and bacterial expression of human Rab31 and Rab32. Mol. Biol. Cell 8, 199a.
  • 11
    Li, G. & Stahl, P.D. (1993) Structure–function relationship of the small GTPase rab5. J. Biol. Chem. 268, 24475–24480.
  • 12
    Goldenring, J.R., Smith, J., Vaughan, H.D., Cameron, P., Hawkins, W. & Navarre, J. (1996) Rab11 is an apically located small GTP-binding protein in epithelial tissues. Am. J. Physiol. 270, G515–G525.
  • 13
    Mustard, J.F., Perry, D.W., Ardlie, N.G. & Packham, M.A. (1972) Preparation of suspensions of washed platelets from humans. Br.␣J. Haematol. 22, 193–204.
    Direct Link:
  • 14
    Fox, J.E.B., Say, A.K. & Haslam, R.J. (1979) Subcellular distribution of the different platelet proteins phosphorylated on exposure of intact platelets to ionophore A23187 or to prostaglandin E1. Possible role of a membrane phosphopolypeptide in␣the regulation of calcium-ion transport. Biochem. J. 184, 651–661.
  • 15
    Yang, T.-T. & Kain, S.R. (1995) Fast hybridization solution for␣the detection of immobilized nucleic acids. Biotechniques 18, 498–503.
  • 16
    Landt, O., Grunert, H.-P. & Hahn, U. (1990) A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene 96, 125–128.
  • 17
    Bhullar, R.P. & Haslam, R.J. (1987) Detection of 23–27 kDa GTP-binding proteins in platelets and other cells. Biochem. J. 245, 617–620.
  • 18
    Schmitt, H.D., Wagner, P., Pfaff, E. & Gallwitz, D. (1986) The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization. Cell 47, 401–412.
  • 19
    Kabcenell, A.K., Goud, B., Northup, J.K. & Novick, P.J. (1990) Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic. J. Biol. Chem. 265, 9366–9372.
  • 20
    Fabiato, A. & Fabiato, F. (1979) Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J.␣Physiol. (Paris) 75, 463–505.
  • 21
    Chavrier, P., Simons, K. & Zerial, M. (1992) The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach. Gene 112, 261–264.
  • 22
    Valencia, A., Chardin, P., Wittinghofer, A. & Sander, C. (1991) The ras protein family: evolutionary tree and role of conserved amino acids. Biochemistry 30, 4637–4648.
  • 23
    Kozak, M. (1991) Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266, 19867–19870.
  • 24
    Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402.
  • 25
    Chen, D., Guo, J., Miki, T., Tachibana, M. & Gahl, W.A. (1996) Molecular cloning of two novel rab genes from human melanocytes. Gene 174, 129–134.
  • 26
    Terryn, N., Anuntalabhochai, S., Van Montagu, M. & Inzé, D. (1992) Analysis of a Nicotiana plumbaginifolia cDNA encoding a novel small GTP-binding protein. FEBS Lett. 299, 287–290.
  • 27
    Jäger, D., Stockert, E., Jäger, E., Güre, A.O., Scanlan, M.J., Knuth, A., Old, L.J. & Chen, Y.-T. (2000) Serological cloning of a melanocyte rab guanosine 5′-triphosphate-binding protein and a chromosome condensation protein from a melanoma complementary DNA library. Cancer Res. 60, 3584–3591.
  • 28
    Norian, L., Dragoi, I.A. & O'Halloran, T. (1999)␣Molecular␣characterization of rabE, a developmentally regulated Dictyostelium homolog of mammalian rab GTPases. DNA Cell Biol. 18, 59–64.
  • 29
    Shimizu, F., Katagiri, T., Suzuki, M., Watanabe, T.K., Okuno, S., Kuga, Y., Nagata, M., Fujiwara, T., Nakamura, Y. & Takahashi, E. (1997) Cloning and chromosome assignment to 1q32 of a human cDNA (RAB7L1) encoding a small GTP-binding protein,␣a␣member of the RAS superfamily. Cytogenet. Cell Genet. 77, 261–263.
  • 30
    Massmann, S., Schürmann, A. & Joost, H.-G. (1997) Cloning of two splicing variants of the novel Ras-related GTPase Rab29 which is predominately expressed in kidney. Biochim. Biophys. Acta 1352, 48–55.
  • 31
    Burstein, E.S. & Macara, I.G. (1992) Interactions of the ras-like protein p25rab3A with Mg2+ and guanine nucleotides. Biochem. J. 282, 387–392.
  • 32
    Pan, J.Y., Sanford, J.C. & Wessling-Resnick, M. (1996) Influence of Mg2+ on the structure and function of Rab5. J. Biol. Chem. 271, 1322–1328.
  • 33
    Olkkonen, V.M., Dupree, P., Killisch, I., Lütcke, A., Zerial, M. & Simons, K. (1993) Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily. J. Cell Sci. 106, 1249–1261.
  • 34
    Kahn, R.A., Der, , C.J. & Bokoch, G.M. (1992) The ras superfamily of GTP-binding proteins: guidelines on nomenclature. FASEB J. 6, 2512–2513.
  • 35
    Moore, I., Schell, J. & Palme, K. (1995) Subclass-specific sequence motifs identified in Rab GTPases. Trends Biochem. Sci. 20, 10–12.
  • 36
    Ostermeier, C. & Brunger, A.T. (1999) Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell 96, 363–374.
  • 37
    Masuda, E.S., Luo, Y., Young, C., Shen, M., Rossi, A.B., Huang, B.C.B., Yu, S., Bennett, M.K., Payan, D.G. & Scheller, R.H. (2000) Rab37 is a novel mast cell specific GTPase localized to secretory granules. FEBS Lett. 470, 61–64.
  • 38
    Brauers, A., Schürmann, A., Massmann, S., Mühl-Zürbes, P., Becker, W., Kainulainen, H., Lie, C. & Joost, H.-G. (1996) Alternative mRNA splicing of the novel GTPase Rab28 generates isoforms with different C-termini. Eur. J. Biochem. 237, 833–840.
    Direct Link:
  • 39
    Stroupe, C. & Brunger, A.T. (2000) Crystal structures of a Rab protein in its inactive and active conformations. J. Mol. Biol. 304, 585–598.DOI: 10.1006/jmbi.2000.4236
  • 40
    Mori, T., Fukuda, Y., Kuroda, H., Matsumura, T., Ota, S., Sugimoto, T., Nakamura, Y. & Inazawa, J. (1999) Cloning and characterization of a novel Rab-family gene, Rab36, within the␣region at 22q11.2 that is homozygously deleted in malignant rhabdoid tumors. Biochem. Biophys. Res. Commun. 254, 594–600.
  • 41
    Chen, W., Feng, Y., Chen, D. & Wandinger-Ness, A. (1998) Rab11 is required for trans-Golgi network-to-plasma membrane transport and a preferential target for GDP dissociation inhibitor. Mol. Biol. Cell 9, 3241–3257.
  • 42
    Ullrich, O., Reinsch, S., Urbé, S., Zerial, M. & Parton, R.G. (1996) Rab11 regulates recycling through the pericentriolar recycling endosome. J. Cell Biol. 135, 913–924.
  • 43
    Casanova, J.E., Wang, X., Kumar, R., Bhartur, S.G., Navarre, J., Woodrum, J.E., Altschuler, Y., Ray, G.S. & Goldenring, J.R. (1999) Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells. Mol. Biol. Cell 10, 47–61.
  • 44
    Urbé, S., Huber, L.A., Zerial, M., Tooze, S.A. & Parton, R.G. (1993) Rab11, a small GTPase associated with both constitutive and regulated secretory pathways in PC12 cells. FEBS Lett. 334, 175–182.
  • 45
    Kessler, A., Tomas, E., Immler, D., Meyer, H.E., Zorzano, A. & Eckel, J. (2000) Rab11 is associated with GLUT4-containing vesicles and redistributes in response to insulin. Diabetologia 43, 1518–1527.DOI: 10.1007/s001250051563
  • 46
    Brondyk, W.H., McKiernan, C.J., Burstein, E.S. & Macara, I.G. (1993) Mutants of Rab3A analogous to oncogenic Ras mutants; sensitivity to Rab3A-GTPase activating protein and Rab3A-guanine nucleotide releasing factor. J. Biol. Chem. 268, 9410–9415.
  • 47
    Stenmark, H., Parton, R.G., Steele-Mortimer, O., Lütcke, A., Gruenberg, J. & Zerial, M. (1994) Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. EMBO J. 13, 1287–1296.
  • 48
    Cormont, M., Bortoluzzi, M.-N., Gautier, N., Mari, M., Van Obberghen, E. & Le Marchand-Brustel, Y. (1996) Potential role of Rab4 in the regulation of subcellular localization of Glut4 in adipocytes. Mol. Cell. Biol. 16, 6879–6886.
  • 49
    Johannes, L., Doussau, F., Clabecq, A., Henry, J.-P., Darchen, F. & Poulain, B. (1996) Evidence for a functional link between Rab3 and the SNARE complex. J. Cell Sci. 109, 2875–2884.
  • 50
    Christoforidis, S., McBride, H.M., Burgoyne, R.D. & Zerial, M. (1999) The Rab5 effector EEA1 is a core component of endosome docking. Nature (London) 397, 621–625.
  • 51
    Klinz, F.-J. (1994) GTP-blot analysis of small GTP-binding proteins. The C-terminus is involved in renaturation of blotted proteins. Eur. J. Biochem. 225, 99–105.
    Direct Link:
  • 52
    Shapiro, A.D., Riederer, M.A. & Pfeffer, S.R. (1993) Biochemical analysis of rab9, a ras-like GTPase involved in protein transport from late endosomes to the trans Golgi network. J. Biol. Chem. 268, 6925–6931.
  • 53
    Simon, I., Zerial, M. & Goody, R.S. (1996) Kinetics of interaction of Rab5 and Rab7 with nucleotides and magnesium ions. J. Biol. Chem. 271, 20470–20478.
  • 54
    Dumas, J.J., Zhu, Z., Connolly, J.L. & Lambright, D.G. (1999) Structural basis of activation and GTP hydrolysis in Rab proteins. Structure 7, 413–423.
View Full Article (HTML) Get PDF (778K)