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High hexokinase activity in tomato fruit perturbs carbon and energy metabolism and reduces fruit and seed size

  1. T. MENU1,†,
  2. P. SAGLIO1,
  3. D. GRANOT2,
  4. N. DAI2,
  5. P. RAYMOND1,
  6. B. RICARD1,*

Article first published online: 26 NOV 2003

DOI: 10.1046/j.0016-8025.2003.01128.x

Issue

Plant, Cell & Environment

Plant, Cell & Environment

Volume 27, Issue 1, pages 89–98, January 2004

Additional Information

How to Cite

MENU, T., SAGLIO, P., GRANOT, D., DAI, N., RAYMOND, P. and RICARD, B. (2004), High hexokinase activity in tomato fruit perturbs carbon and energy metabolism and reduces fruit and seed size. Plant, Cell & Environment, 27: 89–98. doi: 10.1046/j.0016-8025.2003.01128.x

Author Information

  1. 1

    UMR 619 Physiologie et Biotechnologie Végétales, Institut de Biologie Végétale Moléculaire, INRA de Bordeaux, BP 81, F-33883 Villenave d’Ornon Cedex, France and

  2. 2

    Institute of Field and Garden Crops, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel

  1. †

    This paper is dedicated to Thierry Menu (1976–2002), who was in his final year of doctoral studies at the University Victor Segalen Bordeaux 2.

*Bérénice Ricard. Fax: 33 557122541; e-mail: ricard@bordeaux.inra.fr

  • †

    This paper is dedicated to Thierry Menu (1976–2002), who was in his final year of doctoral studies at the University Victor Segalen Bordeaux 2.

Publication History

  1. Issue published online: 26 NOV 2003
  2. Article first published online: 26 NOV 2003
  3. Received 18 July 2003; accepted for publication 18 September 2003

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Keywords:

  • Lycopersicon esculentum;
  • Arabidopsis hexokinase overexpression;
  • carbon metabolism;
  • energy metabolism;
  • fruit development;
  • tomato

ABSTRACT

Tomato (Lycopersicon esculentum var MP-1) plants overexpressing Arabidopsis hexokinase 1 (AtHXK1) exhibited high hexokinase (HXK) activity in correlation with drastic phenotypic modifications in fruit. Transgenic fruit and seeds were reduced in size. Reduction in fruit size was due to decreased cell expansion, which could not be corrected by perfusion with sucrose (Suc). Neither could wild type (WT) fruit and seed size be obtained by grafting of transgenic flowers onto WT shoots. Starch and hexose contents were lower but organic and amino acids were higher in transgenic fruit. Lower respiratory rates measured in vitro accompanied by even lower ATP levels and ATP/ADP ratios indicated metabolic perturbations that may explain, in part, reduced fruit and seed size.

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