Morphological observations on Absidia corymbifera and Absidia blakesleeana strains preserved under mineral oil

  1. Manoel J. S. Santos1,
  2. Pedrina C. de Oliveira2,
  3. Sandra F. B. Trufem3

Article first published online: 17 OCT 2003

DOI: 10.1046/j.0933-7407.2003.00923.x

Issue

Mycoses

Mycoses

Volume 46, Issue 9-10, pages 402–406, October 2003

Additional Information

How to Cite

Santos, M. J. S., de Oliveira, P. C. and Trufem, S. F. B. (2003), Morphological observations on Absidia corymbifera and Absidia blakesleeana strains preserved under mineral oil. Mycoses, 46: 402–406. doi: 10.1046/j.0933-7407.2003.00923.x

Author Information

  1. 1

    Serviço de Micologia Médica do Centro de Pesquisa Clínica Hospital Evandro Chagas–CPqHEC, FIOCRUZ, Rio de Janeiro, RJ

  2. 2

    Departamento de Micologia do Instituto Oswaldo Cruz -IOC-, FIOCRUZ, Rio de Janeiro, RJ

  3. 3

    Instituto de Botânica, Seção de Micologia e Liquenologia, São Paulo, SP, Brasil

*Manoel J. Soares Santos, Serviço de Micologia Médica do Centro de Pesquisa Clínica Hospital Evandro Chagas (CPqHEC), Fundação Oswaldo Cruz (FIOCRUZ), Caixa Postal 926 – CEP: 21045-900, Rio de Janeiro, RJ, Brasil. E-mail: manoel@cpqhec.fiocruz.br

Publication History

  1. Issue published online: 17 OCT 2003
  2. Article first published online: 17 OCT 2003
  3. Accepted for publication 2 July 2002

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Keywords:

  • Absidia;
  • preservation;
  • mineral oil
  • Absidia;
  • Konservierung;
  • Mineralöl

Summary

Macro- and micromorphology of 30 living subcultures of Absidia corymbifera (10 strains plus three strains of Absidia ramosa) and Absidia blakesleeana (two strains) preserved under mineral oil at room temperature for periods ranging from 3 to 44 years in The Fungal Culture Collection of Instituto Oswaldo Cruz (IOC) were observed and described by permanent mycological preparations mounted in a glycerol 10% and/or Amann lactophenol solution. Vegetative and asexual reproductive structures are illustrated by drawings made with the aid of a camera-lucida. The study showed that the period of maintenance under mineral oil and the stress which took place during the period of storage did not affect the vegetative and asexual reproductive morphology of the Absidia strains and species studied here.

Makro- und Mikromorphologie von 30 lebenden Subkulturen von Absidia corymbifera (10 Stämme plus 3 Stämme von Absidia ramosa) und Absidia blakesleeana (2 Stämme),angelegt unter Mineralöl in der Pilzkultursammlung des Oswaldo Cruz Instituts (OCI) bei Raumtemperatur über Zeiträume zwischen 3 und 44 Jahren, wurden an Hand von mikroskopischen Präparaten in 10% igem Glycerol und/oder Amann Laktophenol-Lösung beschrieben. Vegetative und asexuelle Reproduktionsstrukturen werden durch Zeichnungen veranschaulicht, die mittels Camera lucida erstellt wurden. Die Dauer des Aufenthalts unter Mineralöl und der während der Einlagerung aufgetretene Stress beeinflussten nicht die vegetative und asexuelle Reproduktionsmorphologie der hier beobachteten Absidia-Stämme und - Spezies.

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